OX-35 Cell Line from murine myeloma
clone 5, 89041184, Reactivity: Anti- CD4, rat
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About This Item
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biological source
Mouse x Mouse Hybridoma
clone
5, monoclonal
ImmunoDon
BALB/c mouse spleen
Myeloma
NS0/1
Reactivity
Anti-CD4, rat
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a
shipped in
dry ice
Cell Line Origin
Mouse NS0/U x Mouse BALB/c spleen
Cell Line Description
The antibody is directed against the CD4 antigen on rat T cells and macrophages. The specificity is the same as that of W3/25, though their binding is non-competitive. OX-35 will compete with OX-36.
Immunogen
T blasts prepared in mixed lymphocyte reactions with purified rat T helper cells against irradiated spleen
Culture Medium
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Subculture Routine
Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.
Certificates of Analysis (COA)
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