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Key Documents

CV-1

87032605, African green monkey kidney, Fibroblast

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About This Item

UNSPSC Code:
41106514

Product Name

CV-1, from African green monkey kidney, 87032605, Convention on the International Trade in Endangered Species of Wild Fauna and Flora (CITES)

biological source

African green monkey kidney

growth mode

Adherent

karyotype

2n = 60, pseudodiploid

morphology

Fibroblast

receptors

Not specified

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Cell Line Origin

Monkey African Green kidney fibroblast

Cell Line Description

Used in Rous sarcoma virus (RSV) transformation studies. Demonstrates rapid thymidine kinase (TK) activity following induction of simian, adeno and papovavirus infections. Derived from a male adult African green monkey kidney. Cells grow rapidly and form monolayers of fibroblast-like cells that support the growth of SV40. Chromosome number shifts have been observed at high passage levels.

Culture Medium

DMEM (D6546) + 2 mM L-Glutamine (G7513) + 10% FBS / FCS (F2442).

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:6, i.e., seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37 °C.

Other Notes

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