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JU77 cell line human

10092309, human lung, epithelioid: spindly with few vacuoles. Cobblestone morphology at confluency.

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About This Item

UNSPSC Code:
41106514

Product Name

JU77 cell line human, 10092309

biological source

human lung

description

Malignant mesothelioma cells from human lung

growth mode

Adherent

karyotype

Not specified

morphology

Epithelial-like: cells are spindle-shaped with few vacuoles. Upon confluence develop an epithelioid "cobblestone-like mat"

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Cell Line Origin

Human malignant mesothelioma

Cell Line Description

This cell line was derived from the pleural fluid of a patient with malignant mesothelioma. The patient had known exposure to crocidolite asbestos. JU77 is not tumourigenic in nude mice. Cells express cytokeratin and epithelial membrane antigen (EMA) but not mucin. Cells are spindle shaped with few vacuoles. Cell Bank Australia reference number: CBA0140

Application

Investigation of the properties of malignant mesothelioma cells and the development of new diagnostic methods.

Culture Medium

RPMI 1640 (with 2mM L-Glutamine+ 25mM HEPES) + 5% FCS

Subculture Routine

Optimal split ratio 1:8 (seeding density 0.8 x104 cells/cm2).Cells incubated at 37°C with 5% CO2.Harvest the cells using 0.05% Trypsin/EDTA at 37°C for 5 min.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
Depositor and originator: Richard Lake - University of Western Australia, Australia

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