P3X63Ag8.653-TurboDoma
3092502, mouse blood, Lymphoblast
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About This Item
UNSPSC Code:
41106514
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product name
P3X63Ag8.653-TurboDoma, 03092502
biological source
mouse blood
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Mouse BALB/c myeloma, serum-free
Cell Line Description
P3X63Ag8.653 cell line (Sigma Catalogue number. 85011420) adapted to grow in the chemically defined protein- and peptide-free medium TurboDoma™. Suitable for use as a fusion parent for serum-free generation of hybridomas.
Application
Fusion partner for generation of serum-free hybridomas.
Culture Medium
TurboDoma™ medium + 4mM Glutamine. When freezing cells down use TurboDoma medium (50:50 conditioned medium:fresh medium) + 4mM Glutamine + 10% DMSO.
Subculture Routine
Viability may be poor on resuscitation and may initially decrease further. Full recovery may take up to 2 weeks. A centrifugation step to remove the cryoprotectant is essential. Rapidly thaw the frozen ampoule in a water bath at 37°C for 1-2 minutes. Transfer the contents to a centrifuge tube and slowly add 5-10ml of pre-warmed growth media. Remove a sample for counting. Centrifuge at 100g for 2-3 minutes to pellet cells and seed at a relatively high density of 5-7 x 105 cells/ml. Leave culture flask upright and observe regularly until viable proliferating cells are seen. Once established use a split ratio of 1:2 approximately every 4 to 5 days; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
Legal Information
TurboDoma is a trademark of Cell Culture Technologies
Certificates of Analysis (COA)
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