Huh-7D 12 Cell Line human

01042712

• Synonym(s): Huh7 Cells, JTC-39 Cells, Japanese Tissue Culture-39 Cells
• UNSPSC Code: 41106514

Properties

• biological source: human liver
• growth mode: Adherent
• karyotype: That of original Huh-7
• morphology: Epithelial
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• storage temp.: −196°C

Description

General description

Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised. Huh-7D is a derivative of Huh-7 cells, which favor hepatitis C virus replication. Huh-7 cells are superior hepatic cell models compared to HepG2 cells for apolipoprotein based metabolic studies.

Cell Line Origin

Human hepatocellular carcinoma

Cell Line Description

Huh-712D is one of a series of stable HDAg (Hepatitis Delta Antigen)-expressing human hepatoma cell lines that have been isolated and characterised.

Application

Study of intracellular traffic of delta virus antigens and RNA, viral RNA replication and early virus assembly events, and interaction between HDV and HBV.

DNA Profile

STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 10
D16S539: 10
D5S818: 12
D7S820: 11
THO1: 7
TPOX: 8,11
vWA: 16,18

Culture Medium

DMEM (D6546) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)

Subculture Routine

Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin/EDTA; 5% CO₂; 37 C. Media change twice a week

Other Notes

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