C9301

Collagen from chicken sternal cartilage

Type II (Miller), powder, BioReagent, suitable for cell culture

• CAS Number: 9007-34-5
• EC Number: 232-697-4
• MDL number: MFCD00130825
• UNSPSC Code: 12352202
• NACRES: NA.75

Properties

• biological source: chicken (Sternal cartilage)
• Quality Level: 300
• type: Type II (Miller)
• product line: BioReagent
• form: powder
• packaging: glass bottle of 100 mg; poly bottle of 25 mg; glass bottle of 5 mg
• concentration: 60-80% (biuret)
• technique(s): cell culture | mammalian: suitable
• surface coverage: 6‑10 μg/cm²
• solubility: acetic acid: 0.5-2.0 mg/mL (Dissolve for several hours at 2-8 °C, occasionally swirling.)
• UniProt accession no.: P02460
• Binding Specificity: Peptide Source: Fibrinogen
• shipped in: wet ice
• storage temp.: 2-8°C
• Gene Information: chicken ... COL2A1(395069)

Description

General description

Collagen is made of three polypeptide chains. Type 2 collagen is ubiquitously present in the hyaline cartilage. It is a homotrimer made of three α1 chains. Type 2 collagen is also predominant in nucleus pulposus of the vertebral disc and vitreous body of the eye. The proteoglycan obtained from the chicken sternal cartilage is similar to type nine collagen structure.

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.

Application

Collagen from chicken sternal cartilage has been used:

• in enzyme–linked immunosorbent assay (ELISA).
• chondrocyte-mediated tissue production in vitro.
• induction, treatment and assessment of collagenα induced arthritis (CIA).
• cell proliferation assay.
• as a coating for cell culture surfaces.

Biochem/physiol Actions

Collagen breaks down metabolically in the body to release N-telopeptide, which is the N-terminus of collagen. There is also C-telopeptide, which is presumably the C-terminus. N-telopeptide is released in urine, and its detection in diagnostic tests is used to screen for osteoporosis.

Features and Benefits

This collagen has been tested in culture with mammalian cells to verify it is low in endotoxin content.

Other Notes

Major component of articular cartilage.

Preparation Note

Prepared by a modification of the method of Trentham, D.E., et al.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves