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Key Documents

C3545

Sigma-Aldrich

Monoclonal Anti-Calmodulin antibody produced in mouse

clone 6D4, ascites fluid

Synonym(s):

Anti-CALM3, Anti-calmodulin

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About This Item

MDL number:
MFCD00162206
UNSPSC Code:
12352203
NACRES:
NA.44

biological source

mouse

conjugate

unconjugated

antibody form

ascites fluid

antibody product type

primary antibodies

clone

6D4, monoclonal

contains

15 mM sodium azide as preservative

species reactivity

chicken, Dictyostelium discoideum, bovine, rat

technique(s)

western blot: 1:2,000 using a bovine brain calmodulin preparation

isotype

IgG1

UniProt accession no.

P0DP29

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

rat ... Calm1(24242)

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General description

Calmodulin belongs to a family of structurally homologous Ca2+-binding proteins. CALM1 gene is located on human chromosome 14q32.11.
Calmodulin is a highly conserved 17kDa calcium binding protein found in all eukaryotic cells. It is a multifunctional molecule. Monoclonal anti-Calmodulin (mouse IgG1 isotype) is derived from the 6D4 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mice immunized with calmodulin, purified from Dictyostelium discoideum and conjugated to KLH.

Specificity

The antibody recognizes native and SDS-denatured calmodulin.
These antibodies are directed against different epitopes within the calmodulin molecule.

Immunogen

preparation of purified calmodulin from Dictyostelium discoideum conjugated to KLH.

Application

Monoclonal Anti-Calmodulin antibody produced in mouse has been used in:
  • enzyme-linked immunosorbent assay (ELISA)
  • immunoblot
  • dot blot
  • immunocytochemistry
  • immunoprecipitation
  • immunofluorescence

Biochem/physiol Actions

Calmodulin is a ubiquitous molecule which can bind up to 4 calcium ions, regulating and mediating a wide variety of biochemical processes.

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Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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C J Pallen et al.
The Journal of biological chemistry, 259(10), 6134-6141 (1984-05-25)
Calcineurin possesses phosphatase activity towards both protein (Stewart, A.A., Ingebritsen , T.S., Manalan , A., Klee , C.B., and Cohen, P. (1982) FEBS Lett. 137, 80-84) and nonprotein substrates ( Pallen , C.J., and Wang, J.H. (1983) J. Biol. Chem.
The intellectual disability-associated CAMK2G p. Arg292Pro mutation acts as a pathogenic gain-of-function
Proietti OM, et al.
Human Mutation, 39(12), 2008-2024 (2018)
Identification of calmodulin and MlcC as light chains for Dictyostelium myosin-I isozymes
Crawley SW, et al.
Biochemistry, 50(30), 6579-6588 (2011)
Association between the polymorphisms of CALM1 gene and osteoarthritis risk: a meta-analysis based on observational studies
Yang H, et al.
Bioscience Reports, 38(5), BSR20181128-BSR20181128 (2018)
D Hulen et al.
Cell motility and the cytoskeleton, 18(2), 113-122 (1991-01-01)
Monoclonal antibodies were raised against calmodulin purified from Dictyostelium discoideum. To increase its antigenicity, the calmodulin was conjugated to keyhole limpet hemocyanin; mice were immunized with the conjugate. Hybridomas producing antibodies against calmodulin were identified by screening culture supernatants with
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