Skip to Content
Merck
All Photos(1)

Key Documents

C-12530

Sigma-Aldrich

Human Skeletal Muscle Cells (SkMC)

500,000 cryopreserved cells

Synonym(s):

SkMC cells

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41106514
NACRES:
NA.81

biological source

human skeletal muscle

packaging

pkg of 500,000 cells

morphology

(skeletal muscle)

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

General description

Lot specific orders are not able to be placed through the web. Contact your local sales rep for more details.

Cell Line Origin

Muscle

Application

Primary Human Skeletal Muscle Cells (SkMC) are isolated from different skeletal muscles (e.g. Musculus pectoralis major) from adult single donors (lot specific source information is available on request) and are provided in a cryopreserved format. They are positive for sarcomeric myosin and negative for smooth muscle specific α-actin.New skeletal muscle cells originate from quiescent satellite cells, which are located in the muscle fibers between the basal lamina and the sarcolemma. Quiescent satellite cells are activated by stimuli such as muscle damage. After activation, the cells, now called myoblasts, start to proliferate and fuse with damaged muscle fibers or with one another forming new myotubes.SkMC are optimal for in vitro muscle studies. They proliferate very well in the mitogen-rich PromoCell Skeletal Muscle Cell Growth Medium. Fusion to myotubes with typical multinucleated syncytia can be induced by using the PromoCell Skeletal Muscle Cell Differentiation Medium.

Quality

Rigid quality control tests are performed for each lot of Human Skeletal Muscle Cells. They are tested for cell morphology, adherence rate, and cell viability. Immunohistochemical tests for cell-type specific markers are carried out for each lot. Growth performance is tested through multiple passages up to 15 population doublings (PD) under culture conditions without antibiotics and antimycotics. Furthermore, the capacity to differentiate into multinucleated syncytia is routinely checked for each lot. In addition, all cells have been tested for the absence of HIV-1, HIV-2, HBV, HCV, HTLV-1, HTLV-2 and microbial contaminants (fungi, bacteria, and mycoplasma).

Warning

Although tested negative for HIV-1, HIV-2, HBV, HCV, HTLV-1 and HTLV-2, the cells – like all products of human origin – should be handled as potentially infectious. No test procedure can completely guarantee the absence of infectious agents.

Subculture Routine

Click here for more information.

Other Notes

Recommended Plating Density: 3500 - 7000 cells per cm2Passage After Thawing: P2Tested Markers: Sarcomeric myosin positiveDifferentiation capacity to multinucleate syncytia testedGuaranteed population doublings: >15

Recommended products

Recommended Primary Cell Culture Media:Link Link

Disclaimer

RESEARCH USE ONLY. This product is regulated in France when intended to be used for scientific purposes, including for import and export activities (Article L 1211-1 paragraph 2 of the Public Health Code). The purchaser (i.e. enduser) is required to obtain an import authorization from the France Ministry of Research referred in the Article L1245-5-1 II. of Public Health Code. By ordering this product, you are confirming that you have obtained the proper import authorization.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Primary human skeletal muscle cells and culture medium optimized for the in vitro cultivation and differentiation of primary human skeletal muscle cells. Protocols for handling, cell passaging, media, and product use.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service