A9655

Auramine O

Dye content ≥80 %, certified by the Biological Stain Commission

• Synonym(s): 4,4′-(Imidocarbonyl)bis(N,N-dimethylaniline) monohydrochloride, Basic Yellow 2, Pyoctaninum aureum
• Empirical Formula (Hill Notation): C₁₇H₂₁N₃ · HCl
• CAS Number: 2465-27-2
• Molecular Weight: 303.83
• Colour Index Number: 41000
• Beilstein: 4030061
• EC Number: 219-567-2
• MDL number: MFCD00012484
• UNSPSC Code: 12171500
• PubChem Substance ID: 24891484
• NACRES: NA.47

Properties

• grade: certified by the Biological Stain Commission
• Quality Level: 200
• form: powder
• composition: Dye content, ≥80%
• color: yellow to green
• mp: >250 °C (dec.) (lit.)
• solubility: ethanol: 1 mg/mL, yellow to orange
• λmax: 370 nm in H₂O; 432 nm in H₂O
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp
• SMILES string: Cl[H].CN(C)c1ccc(cc1)C(=N)c2ccc(cc2)N(C)C
• InChI: 1S/C17H21N3.ClH/c1-19(2)15-9-5-13(6-10-15)17(18)14-7-11-16(12-8-14)20(3)4;/h5-12,18H,1-4H3;1H
• InChI key: KSCQDDRPFHTIRL-UHFFFAOYSA-N

Description

General description

Certified for use by fluorescence microscopy in Churukian′s modification of Truant′s fluorescent method for acid fast bacilli on paraffin sections.

Auramine O is used for the staining of acid-fast organisms like coccidia. Along with carbol, it generates brilliant yellow fluorochrome of tubercle bacilli. It is effective in detecting positive cases of tuberculosis. Auramine O binds to the mycolic acid in the bacterial cell wall.

Application

Auramine O has been used to study its absorption by a natural biopolymer poly (γ-glutamic acid).

Safety Information

• Pictograms: GHS07,GHS08,GHS09
• Signal Word: Warning
• Hazard Statements: H302 - H319 - H351 - H411
• Precautionary Statements: P201 - P273 - P301 + P312 + P330 - P305 + P351 + P338 - P308 + P313
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Irrit. 2
• Storage Class Code: 6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
• WGK: WGK 3