A9543
Bovine Serum Albumin
heat shock fraction, low endotoxin, pH 7, ≥98%
Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
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About This Item
Recommended Products
biological source
bovine
Quality Level
Assay
≥98%
form
lyophilized powder
mol wt
~66 kDa
purified by
heat shock fractionation
packaging
poly bottle of
origin
USA origin
technique(s)
ELISA: suitable
electrophoresis: suitable
western blot: suitable
impurities
≤1.0 EU/mg endotoxin
loss
≤5%
pH
7
solubility
water: soluble (40 mg/ml)
UniProt accession no.
foreign activity
BT virus, none detected
VSV virus, none detected
storage temp.
2-8°C
Gene Information
bovine ... ALB(280717)
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Application
This A9543 BSA product has been used in various systems, including:
Girbl, T. et al., Immunity, 49(6), 1062-1076.e6 (2018)
- NRK-52E rat kidney epithelioid cells: Ochiai, H. et al., J. Virol., 67(11), 6811-6814 (1993)
- Neonatal porcine pancreatic islets: Ellis, C.E. et al., Islets, 5(5), 216-225 (2013)
- Mouse:
Girbl, T. et al., Immunity, 49(6), 1062-1076.e6 (2018)
- iPSC cell line: Martin, H.C. et al., Science, 362(6419), 1161-1164 (2018).
Biochem/physiol Actions
Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.
Features and Benefits
- Heat shock fractionated
- Low endotoxin
Preparation Note
Prepared using heat shock fractionation
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
Certificates of Analysis (COA)
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