A1603

Alginate Lyase

powder, ≥10,000 units/g solid

• Synonym(s): poly-beta-D-mannuronate lyase
• CAS Number: 9024-15-1
• Enzyme Commission number: 4.2.2.3 (BRENDA, IUBMB)
• MDL number: MFCD03454593
• UNSPSC Code: 12352204
• NACRES: NA.54

Properties

• form: powder
• specific activity: ≥10,000 units/g solid
• storage temp.: 2-8°C

Description

General description

Alginate lyases is present in several marine algae, marine mollusks and a vast range of marine and terrestrial bacteria.

Application

Alginate Lyase has been used:

• in the pre-processing of enzyme
• to de-encapsulate cells
• to digest Pseudomonas exopolysaccharide to facilitate electroporation

Alginate Lyase is used to break down alginate or alginic acid and to reduce viscosity. It is useful during follicle isolation, encapsulation, and culture .

Biochem/physiol Actions

Alginate lyases actively participates in the saccharification of the acidic polysaccharides for the production of bioethanol.

Breaks down alginate or alginic acid to smaller molecules and reduces viscosity.

Unit Definition

One unit will produce an increase the A_235nm of 1.0 per minute per mL of sodium alginate solution at pH 6.3 at 37 °C

Preparation Note

Add 0.15 mI of enzyme solution (1 un/ml) to 4.5 ml of 0.1% sodium alginate (pH 6.3). Incubate at 37 °C for 30 minutes. Terminate reaction by addition of 4.65 ml of 0.1 N NaOH.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves