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92835

Sigma-Aldrich

Atto 550 NHS ester

BioReagent, suitable for fluorescence

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About This Item

MDL number:
MFCD05865403
UNSPSC Code:
12352108
NACRES:
NA.25

product line

BioReagent

Quality Level

100

Assay

≥90.0% (HPLC)
≥90.0% (degree of coupling)

form

powder

manufacturer/tradename

ATTO-TEC GmbH

λ

in ethanol (with 0.1% trifluoroacetic acid)

UV absorption

λ: 553.0-559.0 nm Amax

suitability

suitable for fluorescence

storage temp.

−20°C

Application

Atto fluorescent labels are designed for high sensitivity applications, including single molecule detection. Atto labels have rigid structures that do not show any cis-trans-isomerization. Thus these labels display exceptional intensity with minimal spectral shift on conjugation. Atto 550 NHS ester is a reagent used to label molecules such as proteins with the Atto 550 fluorescence probe. This probe may be used in the design of fluoresence resonance transfer (FRET) detection systems.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Modeling colitis-associated cancer with azoxymethane (AOM) and dextran sulfate sodium (DSS).
Thaker AI, Shaker A, Rao MS, Ciorba MA.
Journal of Visualized Experiments (2012)
Andriy Mokhir et al.
Inorganic chemistry, 44(16), 5661-5666 (2005-08-03)
A concept of fluorescent metal ion sensing with an easily tunable emission wavelength is presented and its principle demonstrated by detection of Cu(2+). A fluorescein dye was chemically modified with a metal chelating group and then attached to the terminus
Smart-aggregation imaging for single molecule localization with SPAD cameras.
Gyongy, I.; et al.
arXiv (2016)
Thorben Cordes et al.
Physical chemistry chemical physics : PCCP, 13(14), 6699-6709 (2011-02-12)
Modern fluorescence microscopy applications go along with increasing demands for the employed fluorescent dyes. In this work, we compared antifading formulae utilizing a recently developed reducing and oxidizing system (ROXS) with commercial antifading agents. To systematically test fluorophore performance in
Sandeep Kumar Vashist
Analytical biochemistry, 421(1), 336-338 (2011-11-19)
A surface plasmon resonance (SPR)-based procedure was developed to determine the effect of antibody modifications on its biomolecular binding behavior. Mouse immunoglobulin G (IgG) was immobilized on a protein A-functionalized gold-coated SPR chip. Goat anti-mouse IgG and its various commercially
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