38132

N-(5-Fluoresceinyl)maleimide

≥90% (HPLC), BioReagent, suitable for fluorescence

• Synonym(s): 5-Maleimido-fluorescein
• Empirical Formula (Hill Notation): C₂₄H₁₃NO₇
• CAS Number: 75350-46-8
• Molecular Weight: 427.36
• MDL number: MFCD00077345
• UNSPSC Code: 12352111
• PubChem Substance ID: 329755743
• NACRES: NA.32

Properties

• product line: BioReagent
• Assay: ≥90% (HPLC)
• fluorescence: λ_ex 490 nm; λ_em 518 nm in 0.1 M Tris pH 8.0
• suitability: corresponds for coupling to thiols; suitable for fluorescence
• storage temp.: 2-8°C
• SMILES string: Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(ccc45)N6C(=O)C=CC6=O)c1
• InChI: 1S/C24H13NO7/c26-13-2-5-17-19(10-13)31-20-11-14(27)3-6-18(20)24(17)16-4-1-12(9-15(16)23(30)32-24)25-21(28)7-8-22(25)29/h1-11,26-27H
• InChI key: AYDAHOIUHVUJHQ-UHFFFAOYSA-N

Description

General description

N-(5-Fluoresceinyl) maleimide, also known as fluorescein-5-maleimide (FM), is a fluorescent derivatization reagent. The pH during the derivatization reaction plays a critical role.

Application

N-(5-Fluoresceinyl) maleimide or fluorescein-5-maleimide is used as a fluorescent biosensor to detect nanoparticles.

N-(5-Fluoresceinyl) maleimide (5-FM) is used to fluorescence label molecules such as proteins and peptides via their thiol groups.

Packaging

Bottomless glass bottle. Contents are inside inserted fused cone.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H302
• Hazard Classifications: Acute Tox. 4 Oral
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves