T7293
Casein Peptone
Enzymatic digest of casein from bovine milk, 11.0-16.0% total nitrogen, suitable for biotechnology and microbiology
Synonym(s):
Peptone from casein, Peptone from casein, Pancreatic digest of casein., Tryptone
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product name
Tryptone, Microbiologically tested.
biological source
bovine milk
Quality Level
form
powder
quality
Microbiologically tested.
packaging
bottle of 1 kg
bottle of 250 g
nitrogen analysis
11.0-16.0% total
loss
≤6% loss on drying
color
light yellow
pH
6.9-7.4
application(s)
food and beverages
microbiology
suitability
Escherichia coli
Haemophilus spp.
Lactobacillus spp.
Streptococcus spp.
coliforms
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General description
Tryptone is a mixture of peptides that is commonly used as a nutrient source in microbiological culture media. It is derived from soybean casein digest and contains a complex mixture of amino acids and peptides, including tryptophan. Tryptone is often used in combination with other ingredients, such as yeast extract and agar, to provide a nutrient-rich environment for the growth of microorganisms. It is particularly useful for culturing fastidious bacteria, which require a more complex nutrient source than simple sugars or amino acids. Tryptone is also used as a component of some enrichment broths and is a common ingredient in commercial media formulations.
Application
Tryptone has a wide range of applications in microbiology, including:
- Growth media: Tryptone is frequently used as a source of nutrients in growth media for bacteria, yeast, and other microorganisms. It can be used alone or in combination with other ingredients, such as agar, glucose, and salts, to provide a complete nutrient source for microbial growth
- Enrichment media: Tryptone is often added to enrichment broths to enhance the growth of slow-growing or stressed bacteria in clinical or environmental samples. This is particularly useful for samples where the target microbe is present in low numbers or is difficult to isolate
- Production of recombinant proteins: Tryptone is a component of many bacterial growth media used to produce recombinant proteins. It provides a rich source of amino acids and peptides, which are needed for protein biosynthesis
- It is also used in microbiological testing to determine the minimum inhibitory concentration (MIC) of antimicrobial agents against test organisms.Overall, the diverse and versatile properties of Tryptone make it a valuable tool in various microbiological applications
Preparation Note
Pancreatic digest of casein.
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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Joseph A Bentil et al.
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In developing countries, local enzyme production can help decrease the dependency of imported enzymes for bioconversion of e.g. cellulosic feedstocks, but the use of conventional nitrogen sources contributes significantly to such enzyme production cost. Use of local resources is therefore
Tarja Kaijalainen et al.
Journal of clinical microbiology, 42(1), 412-414 (2004-01-13)
In STGG (skim milk, tryptone, glucose, glycerol) medium at -80 degrees C, Streptococcus pneumoniae, Haemophilus influenzae, and Moraxella catarrhalis isolates survived for at least 3 years, and the same species have survived in nasopharyngeal swabs for at least 1.5 years.
S Oh et al.
Applied and environmental microbiology, 61(11), 3809-3814 (1995-11-01)
This study was undertaken to find optimum conditions of tryptone, yeast extract, glucose, Tween 80, and incubation temperature for the growth of Lactobacillus casei YIT 9018 and to assess the effects of these factors by use of response surface methodology.
Frederick K Balagaddé et al.
Science (New York, N.Y.), 309(5731), 137-140 (2005-07-05)
Using an active approach to preventing biofilm formation, we implemented a microfluidic bioreactor that enables long-term culture and monitoring of extremely small populations of bacteria with single-cell resolution. We used this device to observe the dynamics of Escherichia coli carrying
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