75482

Gram′s decolorizer solution

for Gram staining

• Synonym(s): Acetone ethanol mixture
• CAS Number: 37348-17-7
• MDL number: MFCD00240361
• UNSPSC Code: 12171500
• NACRES: MA.02

Properties

• Quality Level: 100
• form: liquid
• technique(s): microbe id | staining: suitable
• refractive index: n20/D 1.361
• density: 0.80 g/mL at 20 °C
• antibiotic activity spectrum: Gram-negative bacteria; Gram-positive bacteria
• application(s): diagnostic assay manufacturing; hematology; histology
• storage temp.: room temp

Description

General description

Gram staining is one of the most significant staining techniques in microbiology. Gram′s decolorizer solution is a mixture of ethanol and acetone.

Application

Gram′s decolorizer solution is used in Gram staining procedure, after the iodine step to remove the crystal violet dye. The decolorizer dissolves the lipid layer from the gram-negative cells.

Safety Information

• Pictograms: GHS02,GHS07
• Signal Word: Danger
• Hazard Statements: H225,H319,H336
• Precautionary Statements: P210 - P233 - P240 - P241 - P242 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Flam. Liq. 2 - STOT SE 3
• Target Organs: Central nervous system
• Supplementary Hazards: EUH066
• Storage Class Code: 3 - Flammable liquids
• WGK: WGK 1
• Flash Point(F): 68.0 °F
• Flash Point(C): 20 °C
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves