71007ATMBC

GDS E. coli O157:H7 Tq Automation Kit

BioControl, Molecular based PCR test for the detection of E. coli O157:H7 in food and environmental samples

• Synonym(s): Nucleic acid amplification system
• UNSPSC Code: 41106302

Properties

• Agency: PCR, Immunomagnetic Separation (IMS); AOAC^® (OMA); AOAC^® (PTM); Health Canada; MicroVal
• Quality Level: 200
• shelf life: 6‑12 mo.
• packaging: pkg of 576
• manufacturer/tradename: Assurance GDS^®; BioControl Systems 71007-576ATM
• technique(s): PCR: suitable
• application(s): food and beverages; pathogen testing
• compatibility: BioControl GDS; for use with GDS Rotor-Gene
• storage temp.: 2-8°C
• suitability: Escherichia coli (O157:H7)

Description

General description

GDS E. coli O157:H7 Tq Automation Kit is an automated nucleic acid amplification system for the detection of E. coli O157:H7 in food and environmental samples. The proprietary PickPen^® IMS-based sample preparation procedure quickly and effectively captures E. coli O157:H7, separating the pathogen from inhibitory background materials and ensuring valid PCR reactions for all sample types, including challenging food and environmental samples. Assurance^® GDS PCR technology provides two additional levels of specificity using both highly specific primers and probes to ensure accurate detection of E. coli O157:H7.

Application

GDS for E. coli O157:H7 Tq is an automated nucleic acid amplification system for the detection of pathogenic E. coli O157:H7 in foods, ingredients, and environmental samples.

Features and Benefits

• Results available as fast as 8 hours for 25 g samples / 10 hours for composite 375 g samples
• Positive samples can be confirmed for stx genes in an additional 2 hours with GDS Shiga Toxin Genes (O157) assay (PN 71005BC).
  
• Validated for raw ground beef, raw beef trim, frozen finely textured beef, orange juice, apple juice, fresh vegetables, sprout process water, carcass cloths, among others
• Features innovative PickPen^® immunomagnetic separation (IMS) for additional specificity and fast time to results
• Simplified sample prep does not require heating blocks or preparation of reagents

Components

Each 576ATM (for PPMX Automation) kit includes:

• E. coli O157:H7 Amplification Tubes Tq (0.2 mL)
• O157 Concentration Reagent

For each 576ATM kit, the following are also necessary and should be ordered separately:

• 61031BC Wash Solution Kit
• 34724BC Resuspension Buffer Tq

Compatibility

BioControl GDS

Testing Method

PCR, Immunomagnetic Separation (IMS)

Legal Information

AOAC is a registered trademark of AOAC International

Assurance is a registered trademark of Merck KGaA, Darmstadt, Germany

Assurance GDS is a registered trademark of BioControl Systems, Inc.

PICKPEN is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2