SLM-240

RPMI-1640 complete medium

With ʟ-glutamine and 10% US origin FBS, sterile liquid, suitable for cell cutlure

• Synonym(s): Low Bicarb Formulation, Roswell Park Memorial Institute 1640 medium
• UNSPSC Code: 12352207
• eCl@ss: 32160801
• NACRES: NA.71

Properties

• product name: RPMI 1640 Complete Medium, with 2mM L-Glut and 10% FBS, RPMI 1640 Complete Medium is supplemented with 2mM L-Glutamine and 10% U.S. origin FBS. It is a convenient sterile solution for the culture of a wide variety of cells that grow in suspension or as anchorage dependent, and can be used in fusion protocols and in the growth of hybrid cells.
• Quality Level: 300
• form: liquid
• technique(s): cell culture | mammalian: suitable

Description

General description

RPMI 1640 CompleteMedium is supplemented with 2mM L-Glutamine and 10% U.S.-origin fetal bovineserum (FBS). This sterile solution is convenient to culture a wide range ofcells that grow in suspension or as anchorage dependent. It can also be used infusion protocols and for growing hybrid cells. Roswell Park Memorial Institute(RPMI) 1640 medium can be used for culturing human normal and neoplasticleukocytes. It supports the growth of various cultured cells, like fresh humanlymphocytes in the 72-hour phytohemagglutinin (PHA) stimulation assay if theformulation is properly supplemented.

Application

RPMI 1640 CompleteMedium, with 2mM L-Glut and 10% FBS has been used to culture: Jurkatlymphocytes and human U937 monocyte-like cell line(1), HT-29 and SW480 (humancolon cancer cell line) cells(2), monocytes(3)

Quality

pH= 7.2-7.5
Osmolality= 270-292 mOsm/kg
Endotoxin= ≤2 EU/mL
Mycoplasma= Negative
Sterility Tested= Negative for Growth
Appearance= Orange/Red Clear Liquid
Cell Line Tested= A2780 Human Ovarian Carcinoma

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable