MABC166
Anti-Angiogenin Antibody, clone 26–2F (Azide Free)
clone 26-2F, from mouse
Synonym(s):
Angiogenin, Ribonuclease 5, RNase 5
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About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified antibody
antibody product type
primary antibodies
clone
26-2F, monoclonal
species reactivity
human
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
inhibition assay: suitable
isotype
IgG1κ
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... ANG(283)
General description
Angiogenin (Ang) is a non-glycosylated polypeptide, 123 amino acids in length, with a molecular mass of 14 kDa. It is secreted by tumor cells and is a potent inducer of neovascularization. Angiogenin is produced by a variety of cell types including vascular endothelial cells and smooth muscle cells, fibroblasts, tumor colonic epithelium, normal peripheral blood lymphocytes, lung and colonic epithelial tumor cell lines, and primary gastrointestinal adenocarcinomas. It specifically binds to endothelial cells and elicits second messenger systems. Angiogenin is present in human follicular fluid, and its production is up-regulated by human chorionic gonadotropin and hypoxic conditions. Angiogenin shows a high degree of homology with known ribonucleases such as pancreatic ribonuclease A, and the capacity of angiogenin to induce blood vessel growth is dependent on its ribo-nucleolytic activity. Angiogenin is thought to be involved in the development of solid tumors; angiogenin antagonists are capable of inhibiting tumor growth. Angiogenin is endocytosed by subconfluent endothelial cells and translocated to the nucleus, where it accumulates in the nucleolus. Serum angiogenin concentration is increased in serum of colorectal cancer patients, and correlates with cancer progression. The cytoskeletal protein α-actinin-2 binds to angiogenin, which might be a novel interface target for anti-angiogenesis and anti-tumor therapy.
Immunogen
Purified Human Angiogenin.
Application
Inhibition Assay: A representative lot of this antibody blocked cell-free protein synthesis by angiogenin (Fett et al., (1994) Biochemistry. 33(18):5421-5427).
Inhibition Assay: A representative lot of this antibody inhibited ribonucleolytic activity (Piccoli et al., (1998) Proc. Natl. Acad. Sci. 95:4579–4583).
Immunohistochemistry Analysis: A representative lot of this antibody detected Angiogenin in Neovessels of cancer tissue (Tsuji et al., (2005) Cancer Res. 65:1352-1360).
Immunocytochemistry Analysis: A representative lot of this antibody detected Angiogenin in HeLa and Huvec cells (Tsuji et al., (2005) Cancer Res. 65:1352-1360).
Inhibition Assay: A representative lot of this antibody inhibited ribonucleolytic activity (Piccoli et al., (1998) Proc. Natl. Acad. Sci. 95:4579–4583).
Immunohistochemistry Analysis: A representative lot of this antibody detected Angiogenin in Neovessels of cancer tissue (Tsuji et al., (2005) Cancer Res. 65:1352-1360).
Immunocytochemistry Analysis: A representative lot of this antibody detected Angiogenin in HeLa and Huvec cells (Tsuji et al., (2005) Cancer Res. 65:1352-1360).
Research Category
Apoptosis & Cancer
Apoptosis & Cancer
Research Sub Category
Apoptosis - Additional
Apoptosis - Additional
This Anti-Angiogenin Antibody, clone 26–2F (Azide Free) is validated for use in Immunocytochemistry and Inhibition and Immunohistochemistry for the detection of Angiogenin.
Quality
Evaluated by Immunocytochemistry in HeLa cells.
Immunocytochemistry Analysis: A 4 µg/mL of this antibody detected Angiogenin in Angiogenin-treated and untreated HeLa Cells. HeLa cells that were treated with Angiogenin up-took the protein and translocated it to the nucleus. Untreated HeLa cells did not exhibit any Angiogenin protein signal.
Immunocytochemistry Analysis: A 4 µg/mL of this antibody detected Angiogenin in Angiogenin-treated and untreated HeLa Cells. HeLa cells that were treated with Angiogenin up-took the protein and translocated it to the nucleus. Untreated HeLa cells did not exhibit any Angiogenin protein signal.
Target description
16 kDa calculated
Physical form
Format: Purified
Protein G Purified
Purified mouse monoclonal IgG1κ in buffer containing PBS without preservatives.
Storage and Stability
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Chimeric anti-angiogenin antibody cAb 26-2F inhibits the formation of human breast cancer xenografts in athymic mice.
Piccoli, R, et al.
Proceedings of the National Academy of Sciences of the USA, 95, 4579-4583 (1998)
Takanori Tsuji et al.
Cancer research, 65(4), 1352-1360 (2005-03-01)
Angiogenin is an angiogenic protein known to play a role in rRNA transcription in endothelial cells. Nuclear translocation of angiogenin in endothelial cells decreases as cell density increases and ceases when cells are confluent. Here we report that angiogenin is
A monoclonal antibody to human angiogenin. Inhibition of ribonucleolytic and angiogenic activities and localization of the antigenic epitope.
Fett, J W, et al.
Biochemistry, 33, 5421-5427 (1994)
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