524629
Phosphatase Inhibitor Cocktail Set V, 50X
Phosphatase Inhibitor Cocktail Set V is a 50X cocktail of four serine/threonine and tyrosine phosphatase inhibitors.
Synonym(s):
Phosphatase Inhibitor Mix Set V, Phosphatase inhibitor
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352200
NACRES:
NA.77
Recommended Products
form
liquid
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
protect from light
shipped in
wet ice
storage temp.
−20°C
Related Categories
General description
A cocktail of four phosphatase inhibitors for the inhibition of both serine/threonine and protein tyrosine phosphatases. Available as 1 ml or as a set of five 1 ml vials.
Application
Phosphatase Inhibitor Cocktail Set V, 50X has been used:
- as a supplement in the lysis buffer to dissociate metabolically activated one (MMe) bone marrow macrophages (BMDMs) for Western blot
- in radio-immunoprecipitation assay (RIPA) modified buffer to incubate cultured human umbilical vein endothelial cells (HUVECs) for western blot
- in lysis buffer to lyse cultured HUVECs for immunoprecipitation
- in wash buffer to wash the protein complexes
- as a component in 4-(2-hydroxyethyl) piperazine-1-ethanesulfonic acid (HEPES) buffer to homogenize J774 cells to assess the effect of lipopolysaccharides (LPS) incubation on cytosolic phospholipase A2 (cPLA2) activity
Biochem/physiol Actions
Protein phosphorylation and dephosphorylation play a crucial role in signal transduction, controlling various cellular processes such as cell division, proliferation, and apoptosis. Protein kinases add a phosphate group to specific serine, threonine, or tyrosine residues, which alters the protein′s conformation and its ability to interact with ligands. Protein phosphatases then remove the phosphate group, restoring the protein to its original dephosphorylated state. When extracting phosphorylated proteins from cell and tissue lysates, it is of utmost importance to preserve their phosphorylation state.
Features and Benefits
- Convenient: formulations are ready-to-use, making them easy to use.
- Consistent: high-quality formulations ensure reliable and reproducible performance.
- Flexible: the cocktails are designed to target different categories of phosphatases, offering versatility in experimentation.
Components
Each vial contains 1 ml of aqueous solution with the following components:
- 250 mM Sodium Fluoride
- 50 mM ß-Glycerophosphate (Cat. No. 35675)
- 50 mM Sodium Pyrophosphate Decahydrate
- 50 mM Sodium Orthovanadate
Warning
Toxicity: Toxic (F)
Physical form
In H₂O.
Reconstitution
Following initial thaw, aliquot and freeze (-20°C) for long-term storage. Dilute 1:50 just prior to use.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Eye Irrit. 2
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Umberto Tosi et al.
Science advances, 6(30), eabb4105-eabb4105 (2020-08-25)
Efforts at altering the dismal prognosis of pediatric midline gliomas focus on direct delivery strategies like convection-enhanced delivery (CED), where a cannula is implanted into tumor. Successful CED treatments require confirmation of tumor coverage, dosimetry, and longitudinal in vivo pharmacokinetic
Hongxu Sheng et al.
International journal of molecular medicine, 46(3), 1039-1050 (2020-07-25)
Although significant progress has been made in the treatment of lung cancer, it remains the leading cause of cancer‑associated mortality. Liriopesides B (LPB) is a natural product isolated from the tuber of Liriope platyphylla, whose effective substances have exhibited antitumor activity in
Sonia Sabate-Soler et al.
Glia, 70(7), 1267-1288 (2022-03-10)
The human brain is a complex, three-dimensional structure. To better recapitulate brain complexity, recent efforts have focused on the development of human-specific midbrain organoids. Human iPSC-derived midbrain organoids consist of differentiated and functional neurons, which contain active synapses, as well
Linhai Zhu et al.
International journal of molecular medicine, 48(6) (2021-10-14)
Schizandrin A (SchA) can be extracted from the vine plant Schisandra chinensis and has been reported to confer various biologically active properties. However, its potential biological effects on non‑small cell lung cancer (NSCLC) remain unknown. Therefore, the present study aims to
Fiona Bartoli et al.
The Journal of clinical investigation, 132(5) (2022-01-14)
Piezo1 forms mechanically activated nonselective cation channels that contribute to endothelial response to fluid flow. Here we reveal an important role in the control of capillary density. Conditional endothelial cell-specific deletion of Piezo1 in adult mice depressed physical performance. Muscle
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service