492899
Nuclease, Staphylococcus aureus
Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3′-phosphate groups.
Synonym(s):
Nuclease, Staphylococcus aureus
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About This Item
CAS Number:
MDL number:
UNSPSC Code:
12352202
NACRES:
NA.54
Recommended Products
biological source
Staphylococcus aureus
Quality Level
form
lyophilized solid
specific activity
≥15,000 units/mg protein
manufacturer/tradename
Calbiochem®
storage condition
OK to freeze
desiccated (hygroscopic)
color
off-white
solubility
aqueous buffer: 1 mg/mL
shipped in
ambient
storage temp.
2-8°C
General description
Native Stapylococcus aureus nuclease. Cleaves RNA and DNA to produce oligo- and mononucleotides with terminal 3′-phosphate groups. Also used to convert standard rabbit reticulocyte cell-free extracts (lysate) into mRNA-dependent protein synthesis systems. Note: 1 KU = 1000 units.
Warning
Toxicity: Standard Handling (A)
Unit Definition
One unit is defined as the amount of enzyme that will release a sufficient amount of acid-soluble oligonucleotides to produce an absorbance increase of 1 at 260 nm in 30 min at 37°C.
Other Notes
Krupp, G., and Gross, H.J. 1979. Nucleic Acids Res.6, 3481.
Legal Information
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
Storage Class Code
11 - Combustible Solids
WGK
WGK 3
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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