1.52003

Chromolith^® RP-18 endcapped

L × I.D. 25 mm x 3 mm HPLC column

• Synonym(s): Chromolith^® Flash RP-18 endcapped 25-3 HPLC column
• UNSPSC Code: 41115709

Properties

• material: PEEK column
• Quality Level: 100
• Agency: suitable for USP L1
• product line: Chromolith^®
• feature: endcapped
• packaging: 1 ea of
• extent of labeling: 18% Carbon loading
• parameter: 200 bar pressure; 45 °C max. temp.
• technique(s): HPLC: suitable; LC/MS: suitable; mass spectrometry (MS): suitable (High)
• column L × I.D.: 2.5 cm × 3 mm
• matrix: monolithic silica
• matrix active group: C18 bonding phase
• pore size: 13 nm mesoporosity; 2 μm macroporosity
• operating pH range: 2-7.5
• separation technique: reversed phase
• storage temp.: no temp limit

Description

General description

Chromolith^® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith^® Flash RP-18 endcapped 25-3 columns have macropores with 2 μm diameter, giving a column efficiency exceeding 80,000 plates/meter. The mesopores are 13 nm (130 Å), the surface modification is octadecylsilane with full end-capping. Chromolith^® Flash RP-18 endcapped 25-3 columns fall under the USP L1 Classification. The column efficiency is comparable to a 5 μm particle size fully porous particulate column. Chromolith^® Flash RP-18 endcapped 25-3 columns are perfectly suitable for LC-MS applications.
The column dimension of 25 mm length and 3 mm I.D. is the best choice for rapid separations on any HPLC and UHPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity

The recommended Guard-columns are:
Chromolith^® RP-18 endcapped 5-3 guard cartridges (3 pieces) [1.52005] with the Chromolith^® Guard Cartridge Holder 5 mm x 3 mm [1.52004]
Holder and guard cartridges are also available as Guard cartridge set [1.52008]

The Chromolith^® column coupler can be used to significantly increase the separation efficiency of Chromolith^® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith^® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith^® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Analysis Note

Appearance: passes test
Pressure: ≤ 10 bar
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 80000
Capacity factor (Anthracene): 3.3 - 4.3
Tailing (USP): 0.90 - 1.55 %

Legal Information

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany