07-1401

Anti-GLUT-1 Antibody, CT

from rabbit, purified by affinity chromatography

• Synonym(s): Glucose Transporter type 1, Solute carrier family 2, facilitated glucose transporter, member 1
• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: rabbit
• Quality Level: 100
• antibody form: purified immunoglobulin
• antibody product type: primary antibodies
• clone: polyclonal
• purified by: affinity chromatography
• species reactivity: human
• species reactivity (predicted by homology): mouse (Human. Predicted to react with Mouse based on 100% sequence homology.)
• technique(s): immunocytochemistry: suitable; immunohistochemistry: suitable (paraffin); western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_006516
• UniProt accession no.: P11166
• shipped in: wet ice
• target post-translational modification: unmodified
• Gene Information: human ... SLC2A1(6513)

Description

General description

Solute carrier family 2, facilitated glucose transporter member 1 (UniProt: P11166; also known as Glucose transporter type 1, erythrocyte/brain, GLUT-1, HepG2 glucose transporter) is encoded by the SLC2A1 (also known as GLUT1) gene (Gene ID: 6513) in human. Glucose transporters are a family of integral membrane proteins that facilitative glucose uptake by cells. Seven different glucose transport proteins have been described that are designated as GLUT-1 to 7. GLUT-1 is a highly conserved; ubiquitously distributed, multi-pass membrane protein that is responsible for constitutive glucose uptake. It displays very broad substrate specificity and can transport a wide range of aldoses including both pentoses and hexoses. It is the predominant glucose transporter in embryonic and fetal tissues. In many organs, GLUT-1 is concentrated in endothelial cells of blood-tissue barriers. Hence, it has a specialized role to shuttle glucose between blood and organs that have limited access to small solutes via passive diffusion. It is also abundant in the mammalian erythrocyte membrane where it can rapidly equilibrate glucose between the cytoplasm of the erythrocyte and the blood plasma. GLUT-1 levels are reported to be frequently upregulated during tumorigenesis. Mutations in SLC2A1 gene are linked to GLUT-1 deficiency syndrome 1 and 2 that are characterized by infantile-onset epileptic encephalopathy, delayed development, microcephaly, and motor incoordination, and paroxysmal exercise-induced dyskinesia.

Specificity

This rabbit polyclonal antibody specifically detects Glucose transporter type 1 (GLUT-1). It targets an epitope within 12 amino acids from the C-terminal region.

Immunogen

Epitope: C-Terminus

KLH-conjugated linear peptide corresponding to the C-terminal of Human Glucose transporter-1 (GLUT-1).

Application

Anti-GLUT-1, CT, Cat. No. 07-1401, is a rabbit polyclonal antibody that detects glucose transporter member 1 and is tested for use in Western Blotting, Immunohistochemistry (Paraffin), and Immunocytochemistry

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

Tested Applications

• Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected GLUT-1 in A431 cells.
• Immunohistochemistry (Paraffin) Analysis: A 1:1,000 dilution from a representative lot detected GLUT-1 in Human pancreas, Human lung, and Human placenta tissue sections.
• Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Quality

Evaluated by Western Blotting in Human umbilical vein endothelial cell (HUVEC) lysate.

• Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GLUT-1 in Human umbilical vein endothelial cell (HUVEC) lysate.

Target description

~54 kDa observed; 54.08 kDa calculated. Uncharacterized bands may be observed in some lysate(s).

Linkage

Replaces: AB1340

Physical form

Affinity purified

Purified rabbit polyclonal antibody in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, and 0.1% sodium azide.

Storage and Stability

Recommended storage: +2°C to +8°C.

Analysis Note

Control
Jurkat Cell Lysate

Other Notes

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 2
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable