05-565

Anti-Nucleolin Antibody, clone 3G4B2

culture supernatant, clone 3G4B2, Upstate^®

• UNSPSC Code: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Properties

• biological source: mouse
• Quality Level: 100
• antibody form: culture supernatant
• antibody product type: primary antibodies
• clone: 3G4B2, monoclonal
• species reactivity: human, canine, monkey
• manufacturer/tradename: Upstate^®
• technique(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• isotype: IgG
• NCBI accession no.: NM_005381
• UniProt accession no.: P19338
• shipped in: dry ice
• target post-translational modification: unmodified
• Gene Information: human ... NCL(4691)

Description

General description

Nucleolin, also known as C23, is the major nucleolar protein of exponentially growing eukaryotic cells. It is a ubiquitous conserved multi-domain phosphoprotein found in all vertebrate species. Nucleolin contains a specific bipartite nuclear localization signal sequence and three different structural domains. Nucleolin is a multifunctional protein involved in most steps of ribosome biogenesis. Nucleolin has been implicated in chromatin structure, rDNA transcription, rRNA maturation, ribosome assembly, cytokinesis, nucleogenesis, cell proliferation and growth, and
nucleo-cytoplasmic transport. Nucleolin also exhibits autodegradation, DNA and RNA helicase activities, and DNA-dependent ATPase activity. Nucleolin activities may be regulated by proteolysis, methylation, ADPribosylation, and phosphorylation by several kinases, including casein kinase II (CK2), p34cdc2, and protein kinase C-ζ.

Specificity

nucleolin

Immunogen

Nuclear matrix extract from MDCK cells

Application

Anti-Nucleolin Antibody, clone 3G4B2 is a high quality Mouse Monoclonal Antibody for the detection of Nucleolin & has been validated in IP, WB, ICC.

Quality

routinely evaluated by immunoblot on HeLa nuclear extract

Target description

100kDa

Linkage

Replaces: MABE466

Physical form

mouse culture supernatant containing 0.05% sodium azide prior to the addition of glycerol to 30%

Analysis Note

Control
Positive Antigen Control: Catalog #12-309, Hela cell nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.

Legal Information

UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1