HPLC Analysis of Peptides from BSA on Proteomix® SCX-NP3, Volatile Buffer
Materials
analytical column
Product No.
Description
Pricing
Proteomix® SCX-NP1.7 Strong Cation Exchange Column
L × I.D. 3 cm × 2.1 mm, 1.7 μm particle size, stainless steelCONDITIONS
column
Proteomix SCX-NP1.7, 3 cm x 2.1 mm I.D., 1.7 μm particles (Z777142)
mobile phase
(A) 10 mM sodium phosphate, pH 2.5; (B) A + 1 M NaCl; (C) acetonitrile
gradient
90% A, 0% B, 10% C held for 1.5 min; to 40% A, 50% B, 10% C in 18.5 min
pressure
5511 psi (380 bar)
detector
UV, 214 nm
sample
trypsin digested bovine serum albumin (BSA)
flow rate
0.35 mL/min
Description
Legal Information
Proteomix is a registered trademark of Sepax Technologies