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Key Documents

G005682

HPLC Analysis of Caffeine and Taurine on Ascentis® Express HILIC Column using Evaporative Light Scattering Detection (ELSD)

application for HPLC

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About This Item

technique(s)

HPLC: suitable

test parameters

column: Ascentis Express HILlC, 5 cm x 3.0 mm I.D., 2.7 μm particles (53967-U)
mobile phase: [A] 100 mM ammonium formate, pH 3.0 with formic acid; [B] acetonitrile; (10:90, A:B)
flow rate: 0.6 mL/min
column temp.: 25 °C
detector: ELSD, 55 °C, 3.5 bar nitrogen
injection: 2 μL
sample: 1000 μg/mL in 10:90, water: acetonitrile

suitability

application for HPLC

application(s)

vitamins, nutraceuticals, and natural products

Analysis Note

This application demonstrates the suitability of Ascentis Express HILIC for the efficient separation of caffeine and taurine. Elution is by aqueous normal phase (ANP) as an increase in organic will increase retention.

Legal Information

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Caffeine
    Taurine

analytical column

Product No.
Description
Pricing

standard

Product No.
Description
Pricing

T0625

Taurine, ≥99%

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