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G000595

HPLC Analysis of Tricyclic Antidepressants in Serum on Discovery® C18 after SPE using Discovery® DSC-18

application for SPE, application for HPLC

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About This Item

technique(s)

HPLC: suitable
solid phase extraction (SPE): suitable

test parameters

sample preparation: SPE (Solid Phase Extraction)
SPE tube/cartridge: Discovery DSC-18, 100 mg/1 mL (52602-U)
column: Discovery C18, 15 cm × 4.6 mm, 5 μm preceded by a 2 cm C18 guard column and 0.5 μm frit filter (504955)
mobile phase: [A] acetonitrile: [B] methanol: [C] 25 mM potassium phosphate, monobasic (pH 7 with triethlylamine) (45:25:30, A:B:C)
flow rate: 1 mL/min
column temp.: ambient
detector: UV, 254 nm
injection: 50 μL, diluted porcine serum extract

suitability

application for HPLC
application for SPE

application(s)

clinical

Legal Information

Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany

Analyte

Description
    Nortriptyline
    Doxepin
    Imipramine
    Amitriptyline hydrochloride
    Trimipramine solution

analytical column

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Description
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