807448
TOYOPEARL® HW-40F Bulk Media
phase hydroxylated methacrylate, bottle of 500 mL, 30-60 μm
Synonym(s):
TOYOPEARL® HW-40 Size Exclusion Media
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description
HW-40F
product line
TOYOPEARL®
form
slurry
packaging
bottle of 500 mL
parameter
3 bar max. pressure
technique(s)
HPLC: suitable
matrix
hydroxylated methacrylic polymer
matrix active group
hydroxylated methacrylate phase
particle size
30-60 μm
pore size
100-7000 Da MW range
operating pH
2-13
separation technique
size exclusion (SEC)
General description
Toyopearl HW-40 resin is a hydroxylated methacrylic polymer for size exclusion chromatography. It is typically used for high throughput desalting and buffer exchange. It also can remove surfactants such as Triton X100 from protein solutions. It is often used for smaller biomolecules with exclusion limits up to 10,000 Da.
Application
Toyopearl media fractionate mixtures of proteins and other large molecular weight compounds over a wide size range. Applications include separating RNA from protein, resolving oligosaccharides by degree of polymerization, and isolating agglutinin while maintaining a high hemagglutination titer.
Specifications
Clean in place with 0.5 M NaOH or 0.1 M HCl.
Physical form
Shipped in 20% (v/v) ethanol.
Legal Information
Toyopearl is a registered trademark of Tosoh Corporation
Signal Word
Warning
Hazard Statements
Precautionary Statements
Hazard Classifications
Flam. Liq. 3
Storage Class Code
3 - Flammable liquids
WGK
WGK 1
Flash Point(F)
103.1 °F
Flash Point(C)
39.5 °C
Personal Protective Equipment
dust mask type N95 (US), Eyeshields, Gloves
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