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V3388

Sigma-Aldrich

Vascular Endothelial Growth Factor (aa 207-318) human

≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

Synonym(s):

VEGF112, Vasculotropin

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About This Item

MDL number:
MFCD00286542
UNSPSC Code:
12352202
NACRES:
NA.32

product name

Vascular Endothelial Growth Factor (aa 207-318) human, ≥97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder

biological source

human

Quality Level

100

recombinant

expressed in E. coli

Assay

≥97% (SDS-PAGE)

form

lyophilized powder

potency

0.75-3.75 ng/mL ED50

mol wt

predicted mol wt ~28 kDa

packaging

pkg of 5 μg

storage condition

avoid repeated freeze/thaw cycles

impurities

<1 EU/μg

UniProt accession no.

P15692

storage temp.

−20°C

Gene Information

human ... VEGFA(7422)

Application

Vascular Endothelial Growth Factor (aa 207-318) human also known as VEGF 121 was used for preparing a cellular matrix hybridized with hyaluronic acid (ACM-HA) VEGF constructs which is used in measuring the VEGF-enhanced tissue-engineered bladder graft neovascularization by using dynamic contrast-enhanced MRI. The product was used for creating VEGF-impregnated tissue-engineered construct which was inserted in the critical-sized calvarial defect in the membranous parietal bone of the rabbit for examining and characterizing the angiogenesis necessary for bone formation.

Biochem/physiol Actions

Vascular Endothelial Growth Factor (aa 207-318) human also referred to as VEGF 121 or Vasculotropin is a basic protein that induces endothelial cell growth, vasculogenesis and angiogenesis. VEGF also enhances the growth of endothelial cells isolated from cerebral cortex, fetal and adult aorta as well as human umbilical vein. Cells enhance VEGF 121 secretion from fibrin implants results in local and controlled blood vessel growth.
Potent angiogenesis inducer in vivo.

Other Notes

112 amino acid residue variant of human VEGF.

Physical form

Lyophilized from a 0.2 μm filtered solution in 30% acetronitrile and 0.1% TFA containing 0.25 mg bovine serum albumin.

Analysis Note

The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in human umbilical vein endothelial cells.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Marine Beaumont et al.
NMR in biomedicine, 23(1), 48-55 (2009-08-04)
Tissue engineering is a promising technique for bone repair and can overcome the major drawbacks of conventional autogenous bone grafting. In this in vivo longitudinal study, we proposed a new tissue-engineering paradigm: inserting a biological soft-tissue construct within the bone
Lisa Cartwright et al.
Journal of biomedical materials research. Part A, 77(2), 390-395 (2006-01-24)
Tissue engineered organs require an immediately perfused vascular tree. Currently, neovascularization assessment requires animal sacrifice and graft harvest. In this pilot study we assess whether neovascularization of an engineered urinary bladder construct is enhanced with vascular-derived endothelial growth factor (VEGF)
Leung, D.W., et al.
Science, 246 (1989)
Molecular and biological properties of the vascular endothelial growth factor family of proteins.
N Ferrara et al.
Endocrine reviews, 13(1), 18-32 (1992-02-01)
Martin Ehrbar et al.
Circulation research, 94(8), 1124-1132 (2004-03-27)
Although vascular endothelial growth factor (VEGF) has been described as a potent angiogenic stimulus, its application in therapy remains difficult: blood vessels formed by exposure to VEGF tend to be malformed and leaky. In nature, the principal form of VEGF

Articles

Vascular Endothelial Growth Factors (VEGF)

Vascular endothelial growth factor (VEGF) is a family of closely related growth factors having a conserved pattern of eight cysteine residues and sharing common VEGF receptors. VEGF-A (VEGF) is a potent growth factor for blood vessel endothelial cells, showing pleiotropic responses that facilitate cell migration, proliferation, tube formation, and survival. In the developing embryo VEGF-A mRNA is expressed by cells within tissues undergoing capillarization.

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