SRE0019

Apyrase from potatoes

Suitable for manufacturing of diagnostic kits and reagents, ATPase ≥200 units/mg protein

• Synonym(s): Apyrase from potatoes, Adenosine 5′-diphosphatase, Adenosine 5′-triphosphatase
• CAS Number: 9000-95-7
• Enzyme Commission number: 3.6.1.5 (BRENDA, IUBMB)
• EC Number: 232-569-8
• MDL number: MFCD00130542
• UNSPSC Code: 12352204
• NACRES: NA.32

Properties

• Quality Level: 400
• type: Grade VII
• form: lyophilized powder
• ATPase activity: ≥200 units/mg protein
• secondary activity: ≥50 % of base activity ADPase
• composition: Protein, 25-60%
• solubility: H₂O: soluble 1.0 mg/mL
• application(s): diagnostic assay manufacturing
• foreign activity: Acid Phosphatase ≤2% of base activity
• storage temp.: −20°C

Description

Application

Apyrase is used to hydrolyze nucleoside triphosphates and diphosphates. For hydrolysis of organic di and triphosphates, the optimal pH is 6, and for inorganic substrates, the optimal pH is 5.1. Apyrase, from Sigma, has been used in inhibition studies of platelet-aggregation.

At least two isoenzymes are found in different varieties of S. tuberosum: one with a high ATPase/ADPase ratio (∼10) and another with a low ratio (∼1).
Reaction: ATP → ADP+P_i → AMP+2P_i.

Biochem/physiol Actions

Apyrase is found in all eukaryotes and some prokaryotes. Apyrase, from potato, has a crucial role in regulating growth and development. Apyrase is involved in the inactivation of synaptic ATP as a neurotransmitter following nerve stimulation and in the inhibition of ADP induced platelet aggregation to prevent thrombosis. Divalent metal ions are required for activity and best activity is observed with calcium ion at 5 mM.

Unit Definition

One unit will liberate 1.0 μmole of inorganic phosphate from ATP or ADP per min at pH 6.5 at 30 °C.

Physical form

Partially purified, lyophilized powder containing potassium succinate buffer salts.

Preparation Note

Sold on the basis of ATPase units.

Other Notes

This grade has a low ATPase/ADPase ratio.

Safety Information

• Pictograms: GHS08
• Signal Word: Danger
• Hazard Statements: H334
• Precautionary Statements: P261 - P284 - P501
• Hazard Classifications: Resp. Sens. 1
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable