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Key Documents

SAB5702734

Sigma-Aldrich

Anti-MYL12B Antibody, clone 1X3F1, Rabbit Monoclonal

Synonym(s):

MYL12B, MLC-B, MRLC2, myosin light chain 12B

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About This Item

UNSPSC Code:
12352203

biological source

rabbit

Quality Level

100

material

colorless

clone

1X3F1, monoclonal

form

liquid

mol wt

20 kDa

species reactivity

rat, human, rat

concentration

0.50 mg/mL

technique(s)

immunofluorescence: 1:50 - 1:200
western blot: 1:500 - 1:2000

color

colorless

isotype

IgG

immunogen sequence

MMNEAPGPINFTMFLTMFGEKLNGTDPEDVIRNAFACFDEEATGTIQEDYLRELLTTMGDRFTDEEVDELYREAPIDKKGNFNYIEFTRILKHGAKDKDD

UniProt accession no.

O14950

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... MYL12B(103910)

General description

The activity of nonmuscle myosin II (see MYH9; MIM 160775) is regulated by phosphorylation of a regulatory light chain, such as MRLC2. This phosphorylation results in higher MgATPase activity and the assembly of myosin II filaments (Iwasaki et al., 2001 [PubMed 11942626]).[supplied by OMIM, Mar 2008]

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 73-172 of human MYL12B (O14950).
PBS with 0.02% sodium azide,0.05% BSA,50% glycerol,pH7.3.

Application

WB, IF/ICC

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Certificates of Analysis (COA)

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