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MAK020

Sigma-Aldrich

Fructose-6-Phosphate Assay Kit

sufficient for 100 fluorometric tests

Synonym(s):

Fructose-6-P Quantification Kit

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About This Item

UNSPSC Code:
12161503
NACRES:
NA.84

usage

sufficient for 100 fluorometric tests

detection method

fluorometric

relevant disease(s)

cancer

storage temp.

−20°C

General description

Fructose-6-phosphate (F6P) is a glycolytic pathway intermediate produced by the isomerization of glucose-6-phosphate by phosphoglucose isomerase. F6P is further phosphorylated to fructose 1,6-biphosphate which is subsequently cleaved to glyceraldehyde phosphate and dihydroxyacetone phosphate. F6P can also be shunted from glycolysis to the non-oxidative branch of the pentose phosphate pathway as a means of generative pentose phosphates for nucleotide synthesis. F6P levels are elevated in rapidly proliferating cells such as cancer cells. The Fructose-6-phosphate Assay Kit is a highly sensitive and simple fluorescence-based method of quantifying F6P in a variety of samples.

Application

Fructose-6-Phosphate Assay Kit has been used to measure fructose-6-phosphate (F-6-P) and glucose-6-phosphate (G6P).

Suitability

Suitable for quantifying Fructose-6-Phosphate in a variety of samples

Principle

Fructose-6-phosphate concentration is determined by a coupled enzyme reaction, which results in a fluorometric (λex = 535 nm/λem = 587 nm) product, proportional to the fructose-6-phosphate present. Typical sensitivities for this kit are 0.01 to 0.5 nmoles of F6P.

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

10 - Combustible liquids

Flash Point(F)

188.6 °F - closed cup

Flash Point(C)

87 °C - closed cup

Certificates of Analysis (COA)

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Reciprocal regulation between O-GlcNAcylation and tribbles pseudokinase 2 (TRIB2) maintains transformative phenotypes in liver cancer cells.
Yao B, et al.
Cellular Signalling, 28(11) (2016)
Proteomic and biochemical basis for enhanced growth yield of Enterobacter sp. LCR1 on insoluble phosphate medium.
Kumar A and Rai L C
Microbiological research, 170 (2015)
Lauriane Galle-Treger et al.
The Journal of allergy and clinical immunology, 145(2), 502-517 (2019-11-19)
Allergic asthma is a chronic inflammatory disorder characterized by airway hyperreactivity (AHR) and driven by TH2 cytokine production. Group 2 innate lymphoid cells (ILC2s) secrete high amounts of TH2 cytokines and contribute to the development of AHR. Autophagy is a
Mingliang Wang et al.
Biochemical and biophysical research communications, 504(2), 400-405 (2018-06-13)
This study aims to demonstrate the role of miR-182 in the glucose metabolism of NSCLC cells and the potential mechanism. MTT Cytotoxicity Assay was used to measure the function of differentially expressed miR-182 on two NSCLC cell lines proliferation. Metabolite
Sicheng Gao et al.
DNA and cell biology, 38(4), 286-296 (2019-02-15)
Abnormal expression of O-Linked β-N-acetylglucosamine (O-GlcNAc) and β-catenin is a general feature of cancer and contributes to transformed phenotypes. In this study, we identified the interaction between O-GlcNAc and β-catenin, and explored their effects on the progression of liver cancer.
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