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L2650

Sigma-Aldrich

Lectin from Glycine max (soybean)

peroxidase conjugate, lyophilized powder

Synonym(s):

SBA, Soybean agglutinin

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.32

biological source

Glycine max (soybean)

conjugate

peroxidase conjugate

Assay

85.00-100.00% (Warburg-Christian)

form

lyophilized powder

potency

<80 μg per mL agglutination activity

peroxidase activity

25-100 units/mg protein

composition

Protein, ~95% modified Warburg-Christian

technique(s)

western blot: suitable

UniProt accession no.

P05046

storage temp.

−20°C

Gene Information

soybean ... LE1(100818710)

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Application

General Western Blot Protocol:
  • Glycoprotein sample size: 500ng
  • Lectin Concentration: 0.1ug/ml

  1. Load samples at 500 ng of glycoprotein per lane
  2. Run 4-20% Bis-Tris SDS page gel
  3. Transfer gel to a PVDF membrane
  4. Block membrane for 1 hr at RT with RIPA buffer (R0278 Sigma)
  5. Incubate HRP lectin at 0.1ug/ml with RIPA buffer for 2 hours at RT
  6. Wash membrane 5 x 5 minutes with 25ml RIPA buffer
  7. Detect using chemiluminescent substrate (CPS1-120)

Biochem/physiol Actions

SBA is not blood group specific but has an affinity for N-acetyl-D-galactosamine.

Unit Definition

One unit will form 1 mg purpurogallin in 20 sec from pyrogallol at pH 6.0, at 20 °C.

Physical form

Contains sodium citrate

Preparation Note

Conjugates are prepared from affinity purified lectin.
Prepared from peroxidase type VI (P8375) using a modification of the method of O′Sullivan, which favors low molecular weight conjugates. Repurified after conjugation by affinity chromatography.

Analysis Note

Agglutination activity is expressed in μg/ml and is determined from serial dilutions in phosphate buffered saline, pH 6.8, of a 1 mg per mL solution. This activity is the lowest concentration to agglutinate a 2% suspension of human blood group A erythrocytes after 1 hr incubation at 25 °C.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
019M4106V
11190109
108M4191V
11180541
056M4110V

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C Biel et al.
Archives of toxicology, 96(6), 1815-1827 (2022-04-17)
Human Precision-cut intestinal slices (hPCIS) are used to study intestinal physiology, pathophysiology, drug efficacy, toxicology, kinetics, and metabolism. However, the use of this ex vivo model is restricted to approximately a 24 h timeframe because of declining viability of the hPCIS
A simple method for the preparation of enzyme-antibody conjugates.
M J O'Sullivan et al.
FEBS letters, 95(2), 311-313 (1978-11-15)

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