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GE17-0727-20

SOURCE™ 15RPC

Cytiva 17-0727-20, pack of 10 mL

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About This Item

UNSPSC Code:
13111000
NACRES:
NA.56

ligand

matrix surface

feature

autoclavable 20 min at 121°C

packaging

pack of 10 mL

manufacturer/tradename

Cytiva 17-0727-20

matrix

polystyrene/divinylbenzene

avg. part. size

15 μm (monosized)

cleaning in place

1-14

working range

1-12

capacity

~10 mg binding capacity

suitability

suitable for bioprocess medium

General description

SOURCE™ 15RPC is designed for reversed phase chromatography (RPC) with full pH range stability and suitable for polishing steps in industrial processes requiring high f low velocities and low back-pressure.
~10 mg BSA /ml medium at 300 cm/h, ~30 mg bacitracin/ml medium at 300 cm/h, ~50 mg insulin/ml medium at 300 cm/h

Application

SOURCE™ 15RPC is based on a 15 μm, monosized, rigid polystyrene/divinyl benzene matrix. This gives Low back pressures and high flow rates during operation allowing for high productivity. The 15 μm bead size allows for very high resolution chromatography steps.

High quality assurance standards in combination with the patented production method, particle structure is consistent both bead-to-bead and batch-to-batch.

A controlled pore size distribution and large specific surface area offer excellent capacity for a wide range of molecules, from small peptides and oligonucleotides up to large Proteins. With full pH range stability, SOURCE™ 15RPC is a strong alternative to silica-based RPC matrices.

SOURCE™ 15RPC is available in a range of different bulk pack sizes for easy scale-up and process development. As member of the BioProcess media range, SOURCE™ 15RPC meets industrial demands with security of supply and comprehensive technical and regulatory support.

Features and Benefits

  • Mono-sized 15 μm rigid beads gives Low back pressures and high flow rates allowing for high productivity.
  • Designed for high resolution reversed phase chromatography of Proteins, peptides and oligonucleotides.
  • High chemical stability allowing for wide range of working conditions with good resistance to cleaning conditions at high pH
  • BioProcess medium supported for industrial applications.

Storage and Stability

Please be aware this product may be shipped 90 days before the expiration date. For more information on the batch specific expiration date, please contact technical service.
Store at 4 to 30 °C (20% Ethanol or 70% Acetonitrile)

Analysis Note

To view the Certificate of Analysis for this product, please visit www.cytiva.com.

Legal Information

SOURCE is a trademark of Cytiva

Pictograms

Flame
GHS02

Signal Word

Warning

Hazard Statements

H226

Storage Class Code

3 - Flammable liquids

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Articles

Troubleshooting Reversed Phase Chromatography (RPC)

Poor-quality eluent components can cause a phenomenon referred to as “ghosting”. Trace levels of organic impurities bind to the medium, concentrating during equilibration and sample application. When elution begins, these contaminants appear in the chromatogram as unknown, or “ghost” peaks.

Column Packing and Preparation for Hydrophobic Interaction and Reversed Phased Chromatography

This page discusses column packing and preparation techniques for reverse phase chromatography

HIC as a Capture Step

This page shows Hydrophobic Interaction Chromatography (HIC) in a purification strategy.

Protocols

Reverse Phased Chromatography (RPC) in Practice

In this section the practical aspect of Reverse Phased Chromatography ( RPC) is discussed including media and column selection and eluent selection and preparation.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service