U-87 MG Cell Line human
89081402, human brain (glioblastoma astrocytoma), Epithelial-like
Synonym(s):
U-87MG Cells, U87 Cells, U87MG Cells
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About This Item
UNSPSC Code:
41106514
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U-87 MG Cell Line human, from human brain(glioblastoma astrocytoma), 89081402
biological source
human brain (glioblastoma astrocytoma)
growth mode
Adherent
karyotype
2n = 46
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Cell Line Origin
Human glioblastoma astrocytoma
Cell Line Description
Derived from a malignant glioma from a female patient by explant technique. It is reported to produce a malignant tumour consistent with glioblastoma in nude mice.
Application
Neural studies
U-87 MG cell line has been used to study the effects of the lysosomal destabilizing drug Siramesine on glioblastoma. It has also been used to study calcium-independent disruption of microtubule dynamics by nanosecond pulsed electric fields.
DNA Profile
STR-PCR Data: Amelogenin: X
CSF1PO: 10,11
D13S317: 8,11
D16S539: 12
D5S818: 11,12
D7S820: 8,9
THO1: 9.3
TPOX: 8
vWA: 15,17
CSF1PO: 10,11
D13S317: 8,11
D16S539: 12
D5S818: 11,12
D7S820: 8,9
THO1: 9.3
TPOX: 8
vWA: 15,17
Culture Medium
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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Certificates of Analysis (COA)
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