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biological source
human prostate
growth mode
Adherent
karyotype
Not
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Related Categories
Cell Line Origin
Human prostate normal, serum-free
Cell Line Description
This cell line is a derivative of the normal human prostate cell line PNT2 (Sigma cat no. 95012613) adapted to grow in serum-free medium. PNT2 was established by immortalisation of normal adult prostatic epithelial cells by transfection with a plasmid containing SV40 genome with a defective replication origin. The primary culture was obtained from a prostate of a 33 year old male at post mortem. PNT2 cells contain the SV40 genome and express large T protein.
Culture Medium
Defined Keratinocyte-SFM (Gibco 10785) + Growth Supplement (Gibco 10784-015).
Subculture Routine
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 2-4 x 10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Use Trypsin Inhibitor to inactivate the trypsin. When freezing cells down use conditioned medium plus 10% DMSO.
Other Notes
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Certificates of Analysis (COA)
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