1301 Cell Line human

1051619, human blood, Lymphoblast

• Synonym(s): 1301 Cells
• UNSPSC Code: 41106514

Properties

• Product Name: 1301 Cell Line human, 01051619, T-cell leukemia
• biological source: human blood
• growth mode: Suspension
• karyotype: Tetraploid
• morphology: Lymphoblast
• products: Not specified
• receptors: Not specified
• technique(s): cell culture | mammalian: suitable
• relevant disease(s): cancer
• shipped in: dry ice
• storage temp.: −196°C

Description

Cell Line Origin

Human T-cell leukaemia

Cell Line Description

1301 is a sub-line of CCRF-CEM, Sigma catalogue no. 85112105. It has unusually long telomeres, useful in tests where telomere length is determined, where it can be used as control cells with known telomere length. This cell line has been recommended as a control cell line for use with the Telomere PNA Kit/FITC for flow cytometry from DakoCytomation.

Application

Internal control cell in telomere measurements performed by flow cytometry.

Culture Medium

RPMI 1640 (R0883) + 2mM L-Glutamine (G7513) + 10% FBS / FCS (F2442)

Subculture Routine

Maintain cultures between 3-9 x 100,000 cells/ml; 37°C; 5% CO₂.

Other Notes

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