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C0984

Sigma-Aldrich

Creatine Kinase MB Fraction from human heart

≥70% (SDS-PAGE), liquid

Synonym(s):

CK MB Fraction

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About This Item

MDL number:
UNSPSC Code:
12352204
NACRES:
NA.54

biological source

human heart

Quality Level

Assay

≥70% (SDS-PAGE)

form

liquid

concentration

≥0.5 mg/mL

technique(s)

IR spectroscopy: suitable

suitability

suitable for tissue

UniProt accession no.

application(s)

pharmaceutical

storage temp.

−20°C

Gene Information

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General description

Research area: Cell Signaling
Creatine Kinase (CK) comprises M and B subunit and is a dimeric molecule. It is one among the isoenzymes of CK. The enzyme is majorly present in the myocardium.

Application

Creatine Kinase MB Fraction from human heart has been used:
  • as a diagnostic marker for acute myocardial infarction.
  • to identify overlapping absorbance peaks between blood and the cardiac biomarkers.
  • to study the phosphorylation pathway of dioxolane guanosine (DXG) in vivo, a potential treatment of HIV-1 infection.

Biochem/physiol Actions

Creatine Kinase (CK) catalyzes the formation of creatinine from creatine phosphate. The enzyme is associated with coronary performance. Elevated serum levels of CK-MB are prime indicators of myocardial cellular injury. It is regarded as a “gold standard” for myocardial infarction diagnosis. The levels of cardiac troponin I (cTnI) or cardiac troponin T also correlate to higher CK-MB) levels. CK-MB levels may also be elevated in chronic renal failure.

Components

Solution in 5 mM sodium succinate, 10 mM sodium chloride, 1 mM EDTA, 5 mM β-mercaptoethanol, 50% glycerol, pH 7.0.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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Creatine Kinase
Clinical Methods: The History, Physical, and Laboratory Examinations (1990)
Terry M Welsh et al.
Annals of clinical and laboratory science, 32(1), 44-49 (2002-02-19)
The diagnosis of myocardial infarction (MI) is established in patients with chest pain and equivocal electrocardiogram changes by demonstrating a rise in blood levels of creatine kinase MB (CK-MB) and/or an increase in cardiac troponin I (cTnI) or cardiac troponin
J E Adams et al.
Clinical chemistry, 40(7 Pt 1), 1291-1295 (1994-07-01)
Although measurement of cardiac troponin I (cTnI) is, in some situations, more specific for detection of cardiac injury than is measurement of the MB isoenzyme of creatine kinase (MBCK), its sensitivity and specificity relative to MBCK for detection of myocardial
L Gruberg et al.
The American journal of cardiology, 87(12), 1356-1360 (2001-06-09)
We evaluated the short- and long-term clinical outcomes of 326 consecutive patients with chronic renal failure, not on dialysis, who had creatine kinase (CK)-myocardial band (MB) fraction elevation after successful percutaneous coronary intervention in a native coronary artery. Based on

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