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Key Documents

B8563

Sigma-Aldrich

Aniline Blue solution

2.5% in 2% acetic acid

Synonym(s):

Acid blue 93; Cl 42780

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About This Item

Empirical Formula (Hill Notation):
C32H25N3O9S3Na2
CAS Number:
28631-66-5
Molecular Weight:
737.73
UNSPSC Code:
12352200
NACRES:
NA.47

form

liquid

Quality Level

200

concentration

2.5% in 2% acetic acid

color

dark blue

solubility

water: soluble

λmax

0.21-0.37 at 597-603 nm

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

InChI

1S/C32H27N3/c1-23-22-26(16-21-31(23)33)32(24-12-17-29(18-13-24)34-27-8-4-2-5-9-27)25-14-19-30(20-15-25)35-28-10-6-3-7-11-28/h2-22,34H,33H2,1H3

InChI key

LBLFVSANTXZPEX-UHFFFAOYSA-N

Related Categories

General description

Aniline Blue is the traditional name applied to anionic dyes produced by successive phenylation and sulfonation of basic fuchsine. Aniline blue consists of sulfonation products of variable mixtures of phenylated rosaniline and pararosaniline. It is a mixture of methyl blue and water blue. The dye is also known as cotton blue, and ink blue. Methyl blue of aniline blue belongs to the group of triaminotriphenylmethane dyes.

Application

Aniline blue (also known as cotton blue and ink blue) is a component of commonly used polychrome stains. It is used to stain collagen fibers in tissue sections using Masson′s trichrome protocol for staining multiple components. Collagen is stained blue by this method. The dye is suitable for selective staining of callose in plant specimens and staining histones for assessing nuclear maturity. Heidenhain′s AZAN stain and the Mallory′s polychrome stain both entail staining steps using aniline blue-orange G mixtures. Aniline blue is used in Gomori′s one-step trichrome stain and Mallory′s connective tissue stain for tissue including kidney and intestine.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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