B4261

Biocytin

≥98% (TLC), suitable for drug transporter assays

• Synonym(s): Bct, N_ε-(+)-Biotinyl-L-lysine
• Empirical Formula (Hill Notation): C₁₆H₂₈N₄O₄S
• CAS Number: 576-19-2
• Molecular Weight: 372.48
• Beilstein: 97197
• MDL number: MFCD00077319
• UNSPSC Code: 12352209
• PubChem Substance ID: 24891762
• NACRES: NA.26

Properties

• product name: Biocytin, ≥98% (TLC)
• Assay: ≥98% (TLC)
• form: powder
• optical activity: [α]20/D +53°, c = 1.05% in 0.1 M NaOH(lit.)
• technique(s): drug transporter assay: suitable
• color: white to off-white
• mp: ~245 °C (dec.) (lit.)
• storage temp.: −20°C
• SMILES string: [H][C@]12CS[C@@H](CCCCC(=O)NCCCC[C@H](N)C(O)=O)[C@@]1([H])NC(=O)N2
• InChI: 1S/C16H28N4O4S/c17-10(15(22)23)5-3-4-8-18-13(21)7-2-1-6-12-14-11(9-25-12)19-16(24)20-14/h10-12,14H,1-9,17H2,(H,18,21)(H,22,23)(H2,19,20,24)/t10-,11-,12-,14-/m0/s1
• InChI key: BAQMYDQNMFBZNA-MNXVOIDGSA-N

Description

Application

Biocytin is used as a substrate to study the specificity and kinetics of biotinidase(s), to measure biotinidase deficiency and as a model compound to study various uptake mechanisms of cells and tissues. Biocytin has been used to label neurons, medium spiny neurons and optic nerves.

Biochem/physiol Actions

Biocytin is an adduct of lysine and biotin. It is hydrolyzed by biotinidase, which acts on peptide-incorporated biotin or free biotin. Biocytin acts as a coenzyme.

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves