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B2661

Sigma-Aldrich

Anti-fd Bacteriophage−Biotin antibody produced in rabbit

~3.5 mg/mL, IgG fraction of antiserum

Synonym(s):

Anti-fd Bacteriophage

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.46

biological source

rabbit

Quality Level

conjugate

biotin conjugate

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

concentration

~3.5 mg/mL

technique(s)

indirect ELISA: 1:500-1:1,000 using 1010-1011 phage/ml coated wells

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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Specificity

The antibody binds specifically to phage coat proteins of fd phage or M13 phage and thus may act as a capture antibody when coated directly on multiwell plates or as a primary detection antibody for specifically captured fd or M13 phage. The antibody may be useful in rapidly sorting large phage display libraries (antibody, peptide, etc.) with the expressed proteins fused to either the gene III or to the gene VIII protein of the filamentous phage. The antibody may be used as a reagent in "phage ELISA" offering sensitive and specific activity for detection of recombinant phages.

Immunogen

fd bacteriophage

Application

Anti-fd Bacteriophage-Biotin antibody produced in rabbit was used to access immunoglobulin content using bead-based immunoassays.

Physical form

Solution supplied in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Matthew Robert Tomkins et al.
Sensors (Basel, Switzerland), 15(1), 1047-1059 (2015-01-13)
A detection method that combines electric field-assisted virus capture on antibody-decorated surfaces with the "fingerprinting" capabilities of micro-Raman spectroscopy is demonstrated for the case of M13 virus in water. The proof-of-principle surface mapping of model bioparticles (protein coated polystyrene spheres)
Robert Burger et al.
Lab on a chip, 12(7), 1289-1295 (2012-02-16)
We present a novel centrifugal microfluidic platform for the highly efficient manipulation and analysis of particles for applications in bead-based assays. The platform uses an array of geometrical V-cup barriers to trap particles using stopped-flow sedimentation under highly reproducible hydrodynamic
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