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R1148

Supelco

Rogosa SL agar

suitable for microbiology, NutriSelect® Plus

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About This Item

UNSPSC Code:
41171606
NACRES:
NA.85

sterility

non-sterile

Quality Level

100
200

form

powder

shelf life

limited shelf life, expiry date on the label

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

5.4±0.2 (25 °C)

application(s)

bioburden testing
clinical testing
food and beverages
microbiology

storage temp.

2-8°C

suitability

selective by low pH for Lactobacillus spp.

Related Categories

Application

Used as a selective medium for cultivation of Lactobacilli.

Components

Ingredients (g/L)
Tryptose, 10.00
Yeast extract, 5.00
Dextrose, 10.00
Arabinose, 5.00
Saccharose, 5.00
Sodium acetate, 15.00
Ammonium citrate, 2.00
Monopotassium phoshate, 6.00
Magnesium sulphate, 0.57
Manganese sulphate, 0.12
Ferrous sulphate, 0.03
Polysorbate 80, 1.00
Agar, 15.00

Preparation Note

Suspend 75 g of Rogosa SL Agar in 1000 ml of distilled water. Boil to dissolve the medium completely. Add 1.32 ml of glacial acetic acid (Cat. No. 45726). Mix thoroughly, and distribute into culture tubes or flasks. Heat to 90-100°C for 2-3 minutes. Cool to 45°C for direct inoculation.
DO NOT AUTOCLAVE.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Certificates of Analysis (COA)

Lot/Batch Number
BCCD4881
BCCC2979
BCCB7061
BCBV4642
BCBQ4267V

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A selective medium for the isolation and enumeration of oral and fecal lactobacilli.
M ROGOSA et al.
Journal of bacteriology, 62(1), 132-133 (1951-07-01)

Articles

Microbiological Media and Detection

Molecular biology-based methods, like PCR, can be used for lactobacilli detection.

Culture Method for Beer Quality Control

Traditional methods are based morphology, staining methods, enzyme reactions (metabolism) and diverse media.

Probiotics and Human Health

Today, diverse studies report the benefits of probiotics, such as inhibitory effects on pathogens, aid in the management or prevention of chronic intestinal inflammatory diseases or atopic syndromes, and support to the immune system. Potential beneficial applications abound, researchers continue to evaluate the effictiveness and clarify the mechanisms of action of probiotics.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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