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Key Documents

M6930

Millipore

Mycoplasma enrichment supplement

suitable for microbiology

Synonym(s):

Mycoplasma supplement

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About This Item

UNSPSC Code:
41171614
NACRES:
NA.85

sterility

sterile

Quality Level

form

liquid

shelf life

limited shelf life, expiry date on the label

application(s)

clinical testing
environmental
food and beverages
life science and biopharma
veterinary

microbiology

shipped in

dry ice

storage temp.

−20°C

suitability

mycoplasma

General description

Mycoplasma enrichment supplement is a selective supplement added to the Mycoplasma medium base for the isolation of Mycoplasma. Yeast extract provides the required nutritional needs, minerals, and vitamins. Horse serum is a source of cholesterol and preformed nucleic acid precursors used to promote the growth of cells. Thallium acetate is an antibacterial agent, added to prevent the overgrowth of slow-growing Mycoplasma species bycontaminating organisms. Penicillin inhibits the growth of Gram-positive bacteria in mixed cultures.

Application

Mycoplasma enrichment supplement is recommended as a supplement to Mycoplasma Agar Base and Mycoplasma Broth Base for the isolation and cultivation of Mycoplasma species from clinical specimens.

Components

Composition: (per vial)
Horse serum: 20.00ml
Yeast extract (25% w/v solution): 10.00ml
Thallous acetate: 25.00mg
Penicillin G: 20,000 IU

Reconstitution

Sufficient for 100 ml medium

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Articles

Detect mycoplasma contamination in cell cultures three ways: culture test, DNA stain test, or mycoplasma PCR test. Mycoplasma elimination kits rid cultures of contamination.

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