90554

UVM Listeria Selective Enrichment Broth, modified I & II

suitable for microbiology, NutriSelect^® Plus

• Synonym(s): Listeria Enrichment Medium Base, UVM, University of Vermont Medium, modified
• UNSPSC Code: 41171607
• NACRES: NA.85

Properties

• form: powder
• Quality Level: 200
• shelf life: limited shelf life, expiry date on the label
• composition: beef extract, 5.0 g/L ; casein enzymic hydrolysate, 5.0 g/L ; disodium hydrogen phosphate, 12.0 g/L ; esculin, 1.0 g/L ; monopotassium hydrogen phosphate, 12.0 g/L ; proteose peptone, 5.0 g/L ; sodium chloride, 20.0 g/L ; yeast extract, 5.0 g/L
• manufacturer/tradename: NutriSelect^® Plus
• technique(s): microbiological culture: suitable
• final pH: 7.4±0.2 (25 °C)
• application(s): clinical testing; environmental; food and beverages; pathogen testing; sample preparation; microbiology
• suitability: selective for Listeria spp.

Description

Application

Listeria Enrichment Medium Base (UVM Medium) is used for selective isolation and cultivation of Listeria monocytogenes from clinical specimens.

Preparation Note

Suspend 27.17 grams in 500 ml distilled water. Heat if necessary to dissolve the medium completely. Sterilize by autoclaving at 15 lbs pressure (121°C) for 15 minutes. Cool to 50°C and aseptically add rehydrated contents of 1 vial of Listeria UVM Supplement I (Cat. No. No. 50195) for primary enrichment or 1 vial of Listeria UVM Supplement II (Cat. No. 50279) for secondary enrichment. Mix well and dispense as desired.

Footnote

We offer two media types: the superior granulated GranuCult^® and the cost-efficient powdered NutriSelect^® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany

NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Safety Information

• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 3
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable