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Millipore

Nutrient Broth

Liquid medium used for the cultivation of a wide variety of microorganism, NutriSelect® Plus, suitable for microbiology

Synonym(s):

NB, standard nutrient broth, nutrient broth No.1, Standard - Nutrient Broth

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About This Item

MDL number:
MFCD01867738
UNSPSC Code:
41171607
NACRES:
NA.74

Quality Level

200

sterility

non-sterile

form

powder

shelf life

limited shelf life, expiry date on the label

composition

D(+)-glucose, 1 g/L
peptone, 15 g/L
sodium chloride, 6 g/L
yeast extract, 3 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbiological culture: suitable

final pH

7.5±0.2 (25 °C)

application(s)

agriculture
bioburden testing
environmental
food and beverages
life science and biopharma
microbiology

storage temp.

2-25°C

suitability

Erysipelothrix spp.
nonselective for Brucella spp.
nonselective for Escherichia coli
nonselective for Listeria spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms

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General description

Nutrient Broth is a general-purpose fluid media containing yeast extract, peptone, sodium chloride and other basic nutritional requirements for the growth of a wide variety of microbes that don’t require specific nutrients. It is used in the routine cultivation of microorganisms from both clinical and non-clinical samples. Addition of different biological fluids like blood, serum, egg yolk etc. can make it suitable for cultivation of more fastidious microbes.

Application

•Nutrient Broth No. 1 is used for the routine cultivation of microorganisms not requiring specific nutritional requirements
•It is also used for the enumeration and enrichment of bacteria.
•Nutrient Broth No 1 can be used as a base for preparing special culture media.

Preparation Note

Dissolve 25 g in 1 litre distilled water and fill into tubes. Sterilize by autoclaving at 121°C for 15 minutes.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
BCCG4674
BCCF6854
BCCF2422
BCCC7278
BCBZ9595

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Luciana Maria Saran et al.
Ecotoxicology and environmental safety, 166, 366-374 (2018-10-03)
Soil and water resources must be conserved and protected. However, the management of production activities causes a change in the quality of these natural resources due to accumulation in soil of potentially toxic metals. The objective of the present study
Erica Tirloni et al.
Journal of dairy science, 103(1), 150-160 (2019-11-02)
This study aimed to evaluate the possible inhibitory effect of natural lactic acid bacteria on the growth of 2 Bacillus cereus strains. First, we evaluated the behavior of spores of B. cereus GPe2 and D43 when inoculated before cheesemaking using pasteurized or
Carl-Eric Wegner et al.
Applied and environmental microbiology, 86(1) (2019-10-13)
Methylotrophic bacteria use methanol and related C1 compounds as carbon and energy sources. Methanol dehydrogenases are essential for methanol oxidation, while lanthanides are important cofactors of many pyrroloquinoline quinone-dependent methanol dehydrogenases and related alcohol dehydrogenases. We describe here the physiological
Francisco J Choix et al.
Microbial ecology, 76(2), 430-442 (2018-01-13)
Mutualism interactions of microalgae with other microorganisms are widely used in several biotechnological processes since symbiotic interaction improves biotechnological capabilities of the microorganisms involved. The interaction of the bacterium Azospirillum brasilense was assessed with three microalgae genus, Scenedesmus, Chlorella, and
Tara R Catron et al.
Scientific reports, 9(1), 7064-7064 (2019-05-10)
Estrogenic chemicals are widespread environmental contaminants associated with diverse health and ecological effects. During early vertebrate development, estrogen receptor signaling is critical for many different physiologic responses, including nervous system function. Recently, host-associated microbiota have been shown to influence neurodevelopment.

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