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Millipore

Columbia Agar

NutriSelect® Plus, suitable for microbiology

Synonym(s):

Columbia Blood Agar

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About This Item

UNSPSC Code:
41171605
NACRES:
NA.74

Agency

according to ISO 10272-1:2017
according to ISO 10272-2:2017

Quality Level

sterility

non-sterile

product line

BioChemika

form

powder

shelf life

limited shelf life, expiry date on the label

composition

agar, 15.0 g/L
sodium chloride, 5.0 g/L
special nutrient substrate, 23.0 g/L
starch, 1.0 g/L

manufacturer/tradename

NutriSelect® Plus

technique(s)

microbe id | specific enzyme detection: suitable
microbiological culture: suitable

final pH

7.3±0.2 (25 °C)

application(s)

bioburden testing
clinical testing
cosmetics
environmental
food and beverages
veterinary

microbiology

suitability

Gardnerella spp.
Haemophilus spp.
Pneumococcus spp.
nonselective for Bacteroides spp.
nonselective for Campylobacter spp.
nonselective for Clostridium spp.
nonselective for Corynebacterium spp.
nonselective for Enterococcus spp.
nonselective for Escherichia coli
nonselective for Neisseria spp.
nonselective for Staphylococcus spp.
nonselective for Streptococcus spp.
nonselective for bacteria (General Media)
nonselective for coliforms

Application

This high-grade complete medium acc. to Ellner is for the cultivation of fastidious microorganisms. It is also used as a base for the preparation of a variety of special media.

Preparation Note

Dissolve 42 g in 1 litre distilled water. Sterilize by autoclaving at 121°C for 15 minutes.

Other Notes

Base agar in a medium for isolating Listeria spp. from heavily contaminated material; In a simple plate assay for detection of group A streptococcus proteinase.

Footnote

We offer two media types: the superior granulated GranuCult® and the cost-efficient powdered NutriSelect® culture media, depending on your needs.
The designations basic, plus, or prime are added to indicate the quality control level, from basic quality control to standard QC plus to prime for full regulatory compliance.

Legal Information

GRANUCULT is a registered trademark of Merck KGaA, Darmstadt, Germany
NutriSelect is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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W.L. Hynes et al.
Journal of Microbiological Methods, 4, 25-25 (1985)
E S Bannerman et al.
Applied and environmental microbiology, 54(1), 165-167 (1988-01-01)
Food-associated outbreaks of human listeriosis have emphasized the importance and necessity of screening food for the presence of Listeria isolates-selective agar medium combining acriflavine (10 mg/liter) with ceftazidime (50 mg/liter) was developed. A total of 1,099 cheese production specimens were

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