10745782001
Roche
DNA, lambda
from bacteriophage lambda cl857 Sam 7
Synonym(s):
lambda dna
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About This Item
UNSPSC Code:
41106310
Quality Level
form
solution
mol wt
32,300 kDa
packaging
pkg of 1 mL
manufacturer/tradename
Roche
storage condition
avoid repeated freeze/thaw cycles
concentration
250 μg/mL
impurities
RNA, none detected
nuclease, none detected
color
colorless
solubility
water: miscible
shipped in
dry ice
storage temp.
−20°C (−15°C to −25°C)
Related Categories
General description
The DNA of bacteriophage lambda contains 48,502 nucleotide pairs forming a linear duplex. The 5′ ends of this DNA molecule have self-complementary single-stranded protrusions of 12 nucleotides that can anneal to form circles. Upon entry into the host cell, the DNA circularizes in the presence of DNA ligase. Many such circles are generated during the early lytic infection. At a later stage in replication, these individual circles/rings are converted to linear concatemers by a process called rolling-circle replication. The linear concatemers are cleaved during packaging to form monomeric chromosomal units by the enzyme DNA terminase.
Application
DNA, lambda has been used to spike peripheral blood leukocytes (PBL) before genomic DNA isolation. It has also been used in the preparation of biotinylated double-stranded DNA (dsDNA) substrate.
Quality
The DNA shows the typical cleavage pattern for wild type λDNA in gel electrophoresis after cleavage with restriction endonucleases EcoR I and Hind III.
Sequence
Chain Length 48,502 bp
Physical form
Solution, 250 μg/ml, in 10 mM Tris-HCl, 1 mM EDTA, pH 8.0, ready-to-use
Storage and Stability
Store at -15 to -25°C until use; once thawed, store at 2-8 °C.
Other Notes
For life science research only. Not for use in diagnostic procedures.
Storage Class Code
12 - Non Combustible Liquids
WGK
nwg
Flash Point(F)
No data available
Flash Point(C)
No data available
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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Bacteriophage lambda DNA: the beginning of the end.
A Becker et al.
Journal of bacteriology, 172(6), 2819-2824 (1990-06-01)
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Bekesiova I, et al.
Plant Molecular Biology Reporter, 17, 269-277 (1999)
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Genetic characterization of wild and cultivated plants provides valuable knowledge for conservation and agriculture. DNA sequencing technologies are improving, and costs are dropping. Yet analysis of many species is hindered because they grow in regions that lack infrastructure for advanced
Darren Yang et al.
Nature communications, 7, 11026-11026 (2016-03-18)
We present a miniature centrifuge force microscope (CFM) that repurposes a benchtop centrifuge for high-throughput single-molecule experiments with high-resolution particle tracking, a large force range, temperature control and simple push-button operation. Incorporating DNA nanoswitches to enable repeated interrogation by force
Michael R Wasserman et al.
Cell, 178(3), 600-611 (2019-07-28)
The eukaryotic replicative helicase CMG is a closed ring around double-stranded (ds)DNA at origins yet must transition to single-stranded (ss)DNA for helicase action. CMG must also handle repair intermediates, such as reversed forks that lack ssDNA. Here, using correlative single-molecule
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