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Merck
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Key Documents

03117057001

Roche

Bovine Serum Albumin Fraction V, heat shock, fatty acid free

from bovine serum

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About This Item

UNSPSC Code:
12352106
NACRES:
NA.21

biological source

bovine

Quality Level

Assay

≥98%

form

lyophilized

packaging

pkg of 100 g

manufacturer/tradename

Roche

pH

~7.0

shipped in

wet ice

storage temp.

2-8°C

General description

BSA is a single polypeptide chain consisting of about 583 amino acid residues and no carbohydrates. At pH 5-7, it contains 17 intra-chain disulfide bridges and one sulfhydryl group.

Application

  • Pharmacological assays; ion-binding, transport, or immunological studies
  • Fatty acid and lipid research
  • Stabilization of proteins and enzymes against proteolysis and denaturation

Biochem/physiol Actions

The fatty acids that are insoluble in circulating plasma are transported primarily by serum. The human serum albumin and bovine serum albumin share 76% sequence homology. Being the most abundant protein in the circulatory system, it participates in the maintenance of blood pH and carries several endogenous and exogenous compounds, including fatty acids, metal, amino acids, steroids and drugs.

Quality

Purity: ≥98% albumin (electrophoresis), ≥98% protein
Contaminants: ≤1.2mg total fatty acids/g dry powder, ≤30ppm heavy metals, immunoglobulins are not detectable (by Ouchterlony)

Specifications

Moisture: ≤5%

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Prakrith Vijey et al.
Platelets, 29(6), 583-588 (2018-07-27)
Megakaryocytes (MKs) are specialized precursor cells committed to producing and proliferating platelets. In a cytoskeletal-driven process, mature MKs generate platelets by releasing thin cytoplasmic extensions, named proplatelets, into the sinusoids. Due to knowledge gaps in this process and mounting clinical
Putnam F, et al.
The Plasma Proteins: Structure, Function, and Genetic Control, 5 (2012)
Emilio P Mottillo et al.
Methods in enzymology, 538, 263-278 (2014-02-18)
Intracellular lipolysis is an important cellular process in key metabolic tissues, and while much is known about the enzymatic basis of lipolysis, our understanding of how these processes are organized and regulated within cells is incomplete. Lipolysis takes place on
Rachel D Penrod et al.
Journal of neuroscience methods, 200(1), 1-13 (2011-06-16)
Dendritic spines of striatal Medium Spiny Neurons (MSNs) receive converging dopaminergic and glutamatergic inputs. These spines undergo experience-dependent structural plasticity following repeated drug administration and during disease states like Huntington's and Parkinson's. Thus, understanding the molecular mechanisms leading to structural
Bill X Huang et al.
Journal of the American Society for Mass Spectrometry, 15(8), 1237-1247 (2004-07-28)
Serum albumin is the principal transporter of fatty acids that are otherwise insoluble in circulating plasma. While the crystal structure of human serum albumin (HSA) as well as its binding with fatty acids has been characterized, the three dimensional structure

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