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Key Documents

OP187

Sigma-Aldrich

Anti-EpCAM-1 (Ab-1) Mouse mAb (VU-1D9)

liquid, clone VU-1D9, Calbiochem®

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About This Item

UNSPSC Code:
12352203
NACRES:
NA.41

biological source

mouse

Quality Level

100

antibody form

purified antibody

antibody product type

primary antibodies

clone

VU-1D9, monoclonal

form

liquid

does not contain

preservative

species reactivity

human

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
avoid repeated freeze/thaw cycles

isotype

IgG1

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EPCAM(4072)

General description

Anti-EpCAM-1 (Ab-1), mouse monoclonal, clone VU-1D9, recognizes the ~40 kDa EpCAM-1 in squamous cell carcinoma. It is validated for use in WB, FC, IP, and IHC (frozen and paraffin sections).
Protein A purified mouse monoclonal antibody. Recognizes the ~40 kDa EpCAM-1 protein.
Recognizes the ~40 kDa EpCAM-1 protein in squamous cell carcinoma.

Immunogen

Human
NCI-H69 human small cell lung carcinoma cells

Application

Flow Cytometry (1-3 µg/ml)

Frozen Sections (1-3 µg/ml)

Immunoblotting (1-3 µg/ml)

Immunoprecipitation (1-3 µg/ml)

Paraffin Sections (1-3 µg/ml)

Packaging

Please refer to vial label for lot-specific concentration.

Warning

Toxicity: Standard Handling (A)

Physical form

In PBS.

Reconstitution

Following initial thaw, aliquot and freeze (-20°C)

Analysis Note

Positive Control
Squamous cell carcinoma

Other Notes

Antibody should be titrated for optimal results in individual systems.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Mathilde Dusséaux et al.
Gastroenterology, 153(6), 1647-1661 (2017-08-31)
Hepatitis B virus (HBV) infects hepatocytes, but the mechanisms of the immune response against the virus and how it affects disease progression are unclear. We performed studies with BALB/c Rag2-/-Il2rg-/-SirpaNODAlb-uPAtg/tg mice, stably engrafted with human hepatocytes (HUHEP) with or without
Farhad Khosravi et al.
Nanotechnology, 27(13), 13LT02-13LT02 (2016-02-24)
We demonstrate the rapid and label-free capture of breast cancer cells spiked in buffy coats using nanotube-antibody micro-arrays. Single wall carbon nanotube arrays were manufactured using photo-lithography, metal deposition, and etching techniques. Anti-epithelial cell adhesion molecule (EpCAM) antibodies were functionalized

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