MABE1144
Anti-TET1 Antibody, clone 5D6
clone 5D6, from rat
Synonym(s):
Methylcytosine dioxygenase TET1, CXXC-type zinc finger protein 6, Ten-eleven translocation 1 gene protein homolog
Sign Into View Organizational & Contract Pricing
All Photos(1)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
rat
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
5D6, monoclonal
species reactivity
mouse
technique(s)
ELISA: suitable
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG2aκ
NCBI accession no.
UniProt accession no.
target post-translational modification
unmodified
Gene Information
mouse ... Tet1(52463)
General description
Methylcytosine dioxygenase TET1 (EC 1.14.11.n2; UniProt Q3URK3; also known as CXXC-type zinc finger protein 6, Ten-eleven translocation 1 gene protein homolog) is encoded by the Tet1 (also know as Cxxc6, Kiaa1676) gene (Gene ID 52463) in murine species. TET (ten-eleven translocation) proteins (TET1, TET2, and TET3) oxidize 5-methylcytosine to 5-hydroxymethylcytosine, 5-formylcytosine, and 5-carboxylcytosine, providing a means for active epigenetic DNA demethylation in mammals. TET proteins also modify genomic thymine residues to 5-hydroxyuracil. TET proteins depend on Fe(II) and 2-oxoglutarate as cofactors for activity, and 2-oxoglutarate is known to inhibit their activity, while ascorbate is shown to stimulate TET-mediated cytosine oxidation. In addition, post-translational modifications (PTMs), such as phosphorylation and O-GlcNAcylation, also play a role in regulating the activity of TET proteins. TET1 and TET2 are found highly expressed in mouse embryonic stem cells (mESCs) and TET3 is known to be upregulated in oocytes and oxidize the silenced paternal pronuclear DNA. High levels of TET proteins and genomic 5-hydroxymethylcytosine in neuronal tissues are also reported.
Specificity
Clone 5D6 detected exogenously expressed TET1, but not TET2 or TET3, GST fusion protein (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
Immunogen
Epitope: Region between DNA-binding and 2-oxoglutarate-binding domains
His-tagged recombinant fragment from the region between DNA-binding and 2-oxoglutarate-binding domains of mouse TET1.
Application
Anti-TET1 Antibody, clone 5D6 is an antibody against TET1 for use in Immunocytochemistry, Western Blotting, Immunoprecipitation, ELISA.
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology
Chromatin Biology
Western Blotting Analysis: A representative lot detected the endogenous TET1 in murine embryonic stem cell (mESC) lysate, as well as exogenously expressed TET1, but not TET2 or TET3, GST fusion in lysates from respective HEK293T transfectants (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous TET1 from murine embryonic stem cell (mESC) lysate. Subsequent LC-MS/MS analysis detected the presence of co-immunoprecipitated glycosyltransferase OGT (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
ELISA Anaylsis: Clone 5D6 hybridoma culture supernatant was confirmed for its immunoreactivity against His-tagged murine TET1 by ELISA (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
Immunoprecipitation Analysis: A representative lot immunoprecipitated endogenous TET1 from murine embryonic stem cell (mESC) lysate. Subsequent LC-MS/MS analysis detected the presence of co-immunoprecipitated glycosyltransferase OGT (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
ELISA Anaylsis: Clone 5D6 hybridoma culture supernatant was confirmed for its immunoreactivity against His-tagged murine TET1 by ELISA (Bauer, C., et al. (2015). J. Biol. Chem. 290(8):4801-4812).
Quality
Evaluated by Immunocytochemistry in mouse ESCs.
Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected TET1 in mouse embryonic stem cells (ESCs).
Immunocytochemistry Analysis: A 1:200 dilution of this antibody detected TET1 in mouse embryonic stem cells (ESCs).
Target description
219.3 calculated.
Linkage
Replaces: ABE1034
Physical form
Format: Purified
Protein G purified.
Purified rat monoclonal IgG2aκ antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
Storage and Stability
Stable for 1 year at 2-8°C from date of receipt.
Other Notes
Concentration: Please refer to lot specific datasheet.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Product Selector Tool.
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
Already Own This Product?
Find documentation for the products that you have recently purchased in the Document Library.
Linlin Liu et al.
Frontiers in cell and developmental biology, 9, 644135-644135 (2021-04-10)
Tet enzymes participate in DNA demethylation and play critical roles in stem cell pluripotency and differentiation. DNA methylation alters with age. We find that Tet1 deficiency reduces fertility and leads to accelerated reproductive failure with age. Noticeably, Tet1-deficient mice at
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
Contact Technical Service