MAB5356
Anti-Rhodopsin Antibody, CT, last 9 amino acids, clone Rho 1D4
clone Rho 1D4, Chemicon®, from mouse
Synonym(s):
Anti-CSNBAD1, Anti-OPN2, Anti-RP4
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All Photos(5)
About This Item
UNSPSC Code:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Recommended Products
biological source
mouse
Quality Level
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
Rho 1D4, monoclonal
species reactivity
vertebrates
manufacturer/tradename
Chemicon®
technique(s)
immunocytochemistry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... RHO(6010)
Specificity
Recognizes Rhodopsin. Reacts with both native and recombinant protein. No known reactivity to other proteins.
Immunogen
Bovine rhodopsin
Epitope: C-terminus, last 9 amino acids
Application
Anti-Rhodopsin Antibody, C-terminus, last 9 amino acids, clone Rho 1D4 detects level of Rhodopsin & has been published & validated for use in IC, IH, IP & WB.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Sensory & PNS
Sensory & PNS
Western blot: 1:100-1:500 using ECL. Suggested dilution buffer is PBS and blocking buffer is PBS containing 5% milk. Recognizes 36 kDa monomers, dimmers and trimers depending on sample preparation. Boiling induces the aggregation of rhodopsin.
Western blot of isolated bovine rod outer segment labeled for rhodopsin (~85% of rod outer segment protein) with the rho 1D4 antibody. Amount of rod outer segments applied to the SDS gel: (a) 2.5 mg; (b) 0.63 mg; (c) 0.16 mg; (d) 0.04 mg. At higher protein quantities, dimers, trimers and tetramers of rhodopsin can be observed along with the monomer.
Immunohisto/cytochemistry: 1:100-1:500 on paraformaldehyde and glutaraldehyde fixed frozen tissue sections. Preferred fixation is paraformaldehyde for 4 hours at 2-8°C. Suggested permeabilization method is 0.2% Triton X-100.
Immunoprecipitation
Optimal working dilutions must be determined by the end user.
Western blot of isolated bovine rod outer segment labeled for rhodopsin (~85% of rod outer segment protein) with the rho 1D4 antibody. Amount of rod outer segments applied to the SDS gel: (a) 2.5 mg; (b) 0.63 mg; (c) 0.16 mg; (d) 0.04 mg. At higher protein quantities, dimers, trimers and tetramers of rhodopsin can be observed along with the monomer.
Immunohisto/cytochemistry: 1:100-1:500 on paraformaldehyde and glutaraldehyde fixed frozen tissue sections. Preferred fixation is paraformaldehyde for 4 hours at 2-8°C. Suggested permeabilization method is 0.2% Triton X-100.
Immunoprecipitation
Optimal working dilutions must be determined by the end user.
Target description
48 kDa
Physical form
Format: Purified
Protein A Purified mouse immunoglobulin in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative.
Protein A purified
Storage and Stability
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Analysis Note
Control
Eye
Eye
Other Notes
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Product No.
Description
Pricing
Storage Class Code
10 - Combustible liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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M Keith Wyatt et al.
PloS one, 8(6), e68088-e68088 (2013-07-11)
Age-related macular degeneration (AMD) is a major cause of vision loss. It is associated with development of characteristic plaque-like deposits (soft drusen) in Bruch's membrane basal to the retinal pigment epithelium (RPE). A sequence variant (Y402H) in short consensus repeat
A novel experimental mouse model of retinal detachment: complete functional and histologic recovery of the retina.
Zeng, R; Zhang, Y; Shi, F; Kong, F
Investigative Ophthalmology & Visual Science null
S Nagar et al.
Neuroscience, 160(2), 517-529 (2009-03-11)
Photoreceptor degeneration is followed by significant morphological changes in the second-order retinal neurons in humans and in several genetic animal models. However, it is not clear whether similar changes occur when photoreceptor degeneration is induced nongenetically, raising the question whether
Genetic dissection of TAM receptor-ligand interaction in retinal pigment epithelial cell phagocytosis.
Burstyn-Cohen, T; Lew, ED; Traves, PG; Burrola, PG; Hash, JC; Lemke, G
Neuron null
Jun Yin et al.
Investigative ophthalmology & visual science, 53(8), 4943-4951 (2012-06-30)
In experimental eye research, zebrafish has become a powerful model for human retina disorders. The purpose of the present study is the characterization of antibodies commonly employed in zebrafish models for rod photoreceptor degeneration. The 1D4 monoclonal antibody, developed against
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